Structure and organization of the porcine LCN1 gene encoding Tear lipocalin/von Ebner's gland protein

Gene
P HolzfeindB Redl

Abstract

We have cloned and sequenced the entire genomic fragment of the porcine LCN1 gene which encodes Tear lipocalin/von Ebner's gland protein, a member of the lipocalin superfamily highly expressed in porcine lachrymal and lingual glands. The porcine LCN1 gene is approximately 4.6 kb in size and contains six protein-coding exons and a 3'-nontranslated exon. The structure of this porcine gene is highly similar, in terms of numbers of exons/introns, in size of exons and in intron phasing, to that of the human LCN1 and rat VEGP genes, thus supporting a very close evolutionary relationship of these genes. Within the promoter region of the porcine LCN1 a putative TATA box, a CAAT box and two MRE motifs are found. The same MRE motifs are conserved in the human LCN1 promoter, suggesting that they might be of relevance for LCN1 gene expression. However, additional motifs present in the human LCN1 promoter, such as AP-1 and AP-2 sites, a NF-kappaB site, and a cAMP-responsive element, could not be detected in the porcine LCN1 promoter.

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Citations

Jul 19, 2003·Metabolism: Clinical and Experimental·Olavi UkkolaClaude Bouchard
Nov 4, 2000·Biochimica Et Biophysica Acta·J P Salier
Mar 27, 2001·Nucleic Acids Research·P LichtlenW Schaffner
Nov 4, 2000·Biochimica Et Biophysica Acta·B Redl

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