PMID: 9440045Jan 24, 1998Paper

Structure and other chemical characterizations of gila toxin, a lethal toxin from lizard venom

The Journal of Peptide Research : Official Journal of the American Peptide Society
G Datta, A T Tu

Abstract

The complete primary structure of a lethal toxin, horridum toxin, from the venom of the lizard, Heloderma horridum horridum, was determined by Edman degradation. The amino acid sequence was deduced by overlapping peptide fragments generated by chemical and enzymatic digestions. Horridum toxin causes hemorrhage in internal organs and particularly in the eye, leading to exophthalmia, an effect that has not been observed for other toxins. It is a glycoprotein with a total of 210 residues. Examination of the primary sequence revealed that horridum toxin has considerable homology to tissue-type kallikrein and trypsin. Furthermore, synthetic substrates for trypsin, such as tosyl-L-arginine methyl ester, benzoyl-L-arginine ethyl ester and other p-nitroanilide substrates, were hydrolyzed. The toxin released bradykinin upon hydrolysis of kininogen. This enzymatic behavior is similar to that of plasma kallikrein: however, the presence of a characteristic "kallikrein-like" loop at 91-100 (GTIYNCNYVN) in the primary structure and other features similar to tissue kallikrein suggest that horridum toxin is more like tissue kallikrein. This toxin degraded all three chains of fibrinogen but did not form a clot, which suggests that it is differe...Continue Reading

References

Mar 28, 1991·European Journal of Biochemistry·A VandermeersJ Christophe
Jul 29, 1988·Biochemical and Biophysical Research Communications·Y KomoriH Sugihara
Aug 1, 1986·Annals of Emergency Medicine·J PiacentineP J Ryan
Feb 1, 1986·Postgraduate Medicine·R H Streiffer
Jul 1, 1967·Toxicon : Official Journal of the International Society on Toxinology·R A Patterson
Nov 15, 1981·Analytical Biochemistry·A S EdgeP Weber
Jun 9, 1981·Biochemistry·R A Hendon, A T Tu

❮ Previous
Next ❯

Citations

Jul 16, 2010·Molecular & Cellular Proteomics : MCP·Bryan G FryWayne C Hodgson
Mar 5, 2003·Chembiochem : a European Journal of Chemical Biology·Jon BondebjergMorten Meldal
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Masaki KitaDaisuke Uemura
Apr 8, 2019·Journal of Thrombosis and Thrombolysis·Vance G Nielsen, Nathaniel Frank
Mar 31, 2021·Proceedings of the National Academy of Sciences of the United States of America·Agneesh Barua, Alexander S Mikheyev
Apr 24, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Steven D Aird

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.