PMID: 9547271Jun 20, 1998Paper

Structure and regulation of a polymorphic gene encoding folate receptor type gamma/gamma'

Nucleic Acids Research
H WangManohar Ratnam

Abstract

The human folate receptor (hFR) type gamma and gamma' are constitutively secreted proteins that are expressed primarily in hematopoietic tissues and are potential serum markers for certain hematopoietic malignancies. hFR-gamma' is a variant of hFR-gamma with a two base deletion in its cDNA resulting in a truncated polypeptide. The gene encoding hFR-gamma' was isolated from a placental genomic library. The gene has five exons, four introns and a 5' flanking sequence which contains multiple putative regulatory elements. From RNase protection assay and RACE analysis, the major site of transcriptional initiation was identified at -56 nt. Systematically deleted fragments in the 5' region of the genomic DNA of FR-gamma' were ligated into the PGL3Basic plasmid and the reporter luciferase activity was assayed in cell lysates from transiently transfected NIH3T3 cells. From those results, putative positive and negative regulatory regions in the 5' flanking sequences were noted, and a TATA-less proximal promoter was located between -206 and -22 nt. Gel mobility shift and supershift analyses as well as mutagenesis experiments revealed that Sp1 and ets binding elements in the proximal promoter region confer transcriptional activity. From pa...Continue Reading

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Citations

Jul 16, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ying XuSuoqin Tang
Apr 20, 2004·Advanced Drug Delivery Reviews·Hala Elnakat, Manohar Ratnam
Apr 20, 2004·Advanced Drug Delivery Reviews·Chun-Yen KeMark A Green
Apr 23, 2003·Gene·Karen M M KelleyManohar Ratnam
Aug 29, 2001·Domestic Animal Endocrinology·J L ValletS C Fahrenkrug
Dec 31, 2003·Nuclear Medicine and Biology·Chun-Yen KeMark A Green
Jan 1, 2007·Bioinformation·Kalidoss RamamoorthyRama Shanker Verma
Dec 18, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Yingjuan LuChristopher P Leamon
Sep 29, 2011·Journal of Drug Targeting·Giuseppe TrapaniValentino Laquintana
Mar 6, 2008·Expert Opinion on Drug Delivery·Xiaobin ZhaoRobert J Lee
Jul 2, 2003·Expert Opinion on Biological Therapy·Manohar RatnamXing Pan
May 19, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshiyuki Hattori, Yoshie Maitani
Mar 29, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ouafa Sijilmassi
May 23, 2001·Experimental Biology and Medicine·N M van der PutH J Blom
Mar 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tomonori ShiokawaYoshie Maitani
Mar 24, 2020·Trends in Pharmacological Sciences·Camille AlamReina Bendayan

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