Structure and regulation of KGD1, the structural gene for yeast alpha-ketoglutarate dehydrogenase.

Molecular and Cellular Biology
B Repetto, A Tzagoloff

Abstract

Nuclear respiratory-defective mutants of Saccharomyces cerevisiae have been screened for lesions in the mitochondrial alpha-ketoglutarate dehydrogenase complex. Strains assigned to complementation group G70 were ascertained to be deficient in enzyme activity due to mutations in the KGD1 gene coding for the alpha-ketoglutarate dehydrogenase component of the complex. The KGD1 gene has been cloned by transformation of a representative kgd1 mutant, C225/U1, with a recombinant plasmid library of wild-type yeast nuclear DNA. Transformants containing the gene on a multicopy plasmid had three- to four-times-higher alpha-ketoglutarate dehydrogenase activity than did wild-type S. cerevisiae. Substitution of the chromosomal copy of KGD1 with a disrupted allele (kgd1::URA3) induced a deficiency in alpha-ketoglutarate dehydrogenase. The sequence of the cloned region of DNA which complements kgd1 mutants was found to have an open reading frame of 3,042 nucleotides capable of coding for a protein of Mw 114,470. The encoded protein had 38% identical residues with the reported sequence of alpha-ketoglutarate dehydrogenase from Escherichia coli. Two lines of evidence indicated that transcription of KGD1 is catabolite repressed. Higher steady-sta...Continue Reading

References

Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A J Berk, P A Sharp
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C AlwineG R Stark
Feb 1, 1986·Molecular and Cellular Biology·T M Rickey, A S Lewin
Jun 1, 1986·Molecular and Cellular Biology·K S KimL Guarente
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T Keng, L Guarente
Jan 1, 1985·Molecular & General Genetics : MGG·M E Spencer, J R Guest
May 1, 1974·Archives of Biochemistry and Biophysics·P S Perlman, H R Mahler
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Laughon, R F Gesteland
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Oct 3, 1983·European Journal of Biochemistry·P E StephensJ R Guest
Jun 1, 1984·European Journal of Biochemistry·M G DarlisonJ R Guest
Oct 1, 1952·The Biochemical Journal·E C SLATER, W D BORNER

❮ Previous
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Citations

Jun 26, 2007·International Journal of Medical Microbiology : IJMM·Harald KuschMichael Hecker
Aug 22, 2006·Biochimica Et Biophysica Acta·Charles E MartinYide Jiang
Dec 3, 2014·Advances in Biological Regulation·Bola OlayanjuMichael Hampsey
Jul 23, 1999·BioFactors·J R Schultz, C F Clarke
Sep 22, 1992·Biochemistry·B SumegiP A Srere
Jan 1, 1995·Journal of Basic Microbiology·Y GbelskáJ Subík
Aug 29, 2014·Molecular Biology of the Cell·Manfred HeubleinMartin Ott
Feb 4, 2011·Molecular Biology of the Cell·George S DavidsonMargaret Werner-Washburne
Jan 15, 2002·The Journal of Biological Chemistry·Carlos Santos-OcañaCatherine F Clarke
Jan 1, 1996·Folia Microbiologica·P Haviernik, G J Laquin
Aug 13, 2011·Journal of Bioenergetics and Biomembranes·Erich B TaharaAlicia J Kowaltowski
Jan 24, 2020·Yeast·Thomas PerliJean-Marc Daran
Mar 13, 2015·G3 : Genes - Genomes - Genetics·Leandro José de AssisNeil Andrew Brown
Aug 1, 1995·Molecular and Cellular Biochemistry·B J Nichols, R M Denton
Sep 11, 1989·FEBS Letters·C F HawkinsR N Perham
Jan 1, 1995·Progress in Biophysics and Molecular Biology·M A Aon, S Cortassa
Oct 20, 2005·Journal of Bioscience and Bioengineering·Y ArikawaM Okazaki

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