PMID: 8980512Nov 1, 1996Paper

Structure and regulation of the maize Bronze2 promoter

Plant Molecular Biology
J P Bodeau, V Walbot

Abstract

The maize Bronze2 (Bz2) gene encodes a glutathione S-transferase that is required for anthocyanin pigment accumulation. Two classes of regulatory proteins, R and C1, are required for transcriptional activation of Bz2 and several additional structural genes. Functional domains of the Bz2 promoter were identified using Bz2 promoter-driven luciferase reporter genes electroporated into maize protoplasts together with R and C1 expression plasmids. Complete regulation was conferred by 224 nt of the Bz2 promoter. Within this region at least two separable regions are independently capable of conferring regulation by R and C1. Predicted regulatory elements corresponding to two classes of sequence motifs, the Myb-box homologous 'C1-motif', TAACTG/CAGTTA, and the G-box and E-box homologous 'R-motif', CACGTG, were shown to be important for full R and C1 activation of the Bz2 promoter. Expression of reconstructed Bz2 genes with mutated promoters was quantified using RNase protection, and this analysis confirmed results obtained using reporter genes.

References

Jan 1, 1992·Methods in Enzymology·K R LuehrsenV Walbot
Jan 1, 1992·Analytical Biochemistry·W P Deng, J A Nickoloff
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J HershbergerV Walbot
Jan 1, 1991·Annual Review of Genetics·H K DoonerR A Jorgensen
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E GrotewoldT Peterson
Dec 1, 1987·Genes & Development·J CallisV Walbot
Oct 27, 1988·Nature·H BiedenkappK H Klempnauer
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R A JeffersonD Hirsh
Dec 1, 1994·Plant Molecular Biology·J C Carle-UriosteV Walbot
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T M KleinM E Fromm

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Citations

Jul 17, 1999·Trends in Plant Science·O A OlsenS E Nichols
Mar 6, 1999·Current Opinion in Plant Biology·B Weisshaar, G I Jenkins
Jun 23, 2004·The Plant Cell·Christopher Dean GoodmanVirginia Walbot
Jun 3, 2000·Annual Review of Immunology·D Nemazee
Jun 3, 2000·Annual Review of Immunology·S D FugmannD G Schatz

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