Structure and viability of 24- and 72-h-old biofilms formed by four pathogenic bacteria on polystyrene and glass contact surfaces

Food Microbiology
Cristina Rodríguez-MelcónCarlos Alonso-Calleja

Abstract

The biofilms formed by Salmonella Hadar (SH174), Listeria monocytogenes (LM6), methicillin-resistant Staphylococcus aureus (MRSA125) and vancomycin-resistant Enterococcus faecium (VRE61s) on polystyrene and glass after 24 h and 72 h of incubation at 37 °C were examined by confocal laser scanning microscopy (CLSM) after staining with SYTO9 and propidium iodide (PI). A lower average biovolume (P < 0.05) was observed for SH174 biofilms (73,073.61 ± 52,365.90 μm3 in the observation field of 14,161 μm2) than for LM6 (180,804.50 ± 32,554.62 μm3), MRSA125 (208,605.34 ± 57,534.93 μm3) and VRE61s (212,543.91 ± 39,718.62 μm3) biofilms. SH174 showed the greatest (P < 0.05) biovolume on glass, as compared with polystyrene. Biofilms of LM6, MRSA125 and VRE61s were produced at comparable levels on both contact surfaces. After 24 h, SH174 formed small scattered cell clusters, and biovolume of biofilms increased (P < 0.05) after 72 h. By contrast, LM6, MRSA125 and VRE61s formed compact biofilms quickly (24 h) on both contact surfaces. Seventy-two-hour-old biofilms showed the largest biovolumes of dead or damaged (PI-stained) cells, except for MRSA125 (polystyrene) and VRE61s (polystyrene and glass). Appropriate procedures for the disinfection ...Continue Reading

Citations

Dec 12, 2018·Foodborne Pathogens and Disease·Cristina Rodríguez-MelcónCarlos Alonso-Calleja
Jun 15, 2019·International Journal of Environmental Research and Public Health·Yuman Zhou, Xiaorong Gao
Sep 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alex Fiori SilvaManuel Simões
Dec 20, 2018·Frontiers in Microbiology·David Rodríguez-LázaroMarta Hernández
Feb 18, 2021·Future Microbiology·Lei YuanZhenquan Yang

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