PMID: 8612071May 1, 1996Paper

Structure based design and characterization of peptides that inhibit IgE binding to its high-affinity receptor

Nature Structural Biology
J M McDonnellB J Sutton

Abstract

We have designed synthetic peptide inhibitors of the interaction between IgE and its high affinity receptor, Fc epsilon RI. The structure of the second domain of CD2 was used as a modelling template for the second alpha-chain domain of Fc epsilon RI, the C-C' loop of which has been implicated in the interaction with IgE. An L-amino acid peptide and a retro-enantiomeric D-amino acid peptide were designed to mimic the conformation of the C-C' region. Both peptides were cyclized by disulphide bond formation between terminal cysteine residues, and show mirror image symmetry by circular dichroism analysis. The C-C' peptide mimics act as competitive inhibitors of IgE binding. The cyclic L- and retro D-peptides exhibited KDs of approximately 3 microM and 11 microM, respectively, for IgE. Further, the peptides inhibit IgE-mediated mast cell degranulation, an in vitro model of an allergic response.

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S ChenM I Greene
Jan 1, 1992·Annual Review of Biochemistry·J Rizo, L M Gierasch
Sep 16, 1992·Biochemical and Biophysical Research Communications·M SatoM Kahn
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H NakanishiM Kahn
Jun 1, 1989·European Journal of Immunology·S SchwarzbaumZ Eshhar
Jan 1, 1985·Advances in Protein Chemistry·G D RoseJ A Smith
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A ShimizuP Leder
Jan 1, 1986·Annual Review of Immunology·H MetzgerR Quarto
Jan 1, 1988·Advances in Protein Chemistry·W F Degrado
Jan 1, 1988·Biopolymers·R C HiderN Tamiya
Jan 1, 1987·Biopolymers·A HäberliW Känzig
Jul 1, 1969·Angewandte Chemie·M M ShemyakinV T Ivanov
Jan 20, 1995·Science·T Clackson, J A Wells
Jan 1, 1994·Advances in Immunology·M D Hulett, P M Hogarth
Sep 30, 1994·Journal of Molecular Biology·P BorkC Sander
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·G GuichardS Muller
Dec 2, 1993·Nature·B J Sutton, H J Gould
Nov 1, 1978·Analytical Biochemistry·C T ChangJ T Yang

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Citations

Aug 1, 1997·Current Opinion in Biotechnology·T Kieber-EmmonsM I Greene
Aug 28, 1998·Current Opinion in Biotechnology·M H Van Regenmortel, S Muller
Aug 28, 1999·Bioorganic & Medicinal Chemistry Letters·M TakahashiH Mihara
May 9, 2012·Nature Medicine·Stephen J Galli, Mindy Tsai
Apr 26, 2011·Nature Structural & Molecular Biology·Mary D HoldomBrian J Sutton
Jun 8, 1999·Annual Review of Immunology·J P Kinet
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·J P BriandS Muller
Jul 27, 2014·Current Opinion in Pharmacology·Francesco BorrielloGianni Marone
Aug 8, 2013·Future Medicinal Chemistry·Lucy D SmithAlan C Spivey
Jul 5, 2005·Expert Opinion on Investigational Drugs·S MullerF Brown
Aug 25, 2009·Molecular Immunology·Annamaria SandomenicoMenotti Ruvo
Dec 20, 2015·Cytokine & Growth Factor Reviews·Mathieu Nadeau-ValléeSylvain Chemtob
Aug 9, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·A BukuM Mezei
Jul 16, 2008·Chemical Biology & Drug Design·Angeliki BukuJoseph A Price
Feb 5, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Nicolas BoutardWilliam D Lubell
Jun 11, 2002·Biochemical and Biophysical Research Communications·Akio IwasakiTakao Nagoya
Mar 11, 2005·Expert Opinion on Therapeutic Targets·Akira NakamuraToshiyuki Takai
Jul 2, 2015·European Journal of Pharmacology·Ulrich BlankMarc Benhamou
Aug 4, 2007·Molecular Immunology·Angela C CendronP Mark Hogarth
Jan 6, 1999·Cell·S C GarmanT S Jardetzky
Mar 4, 2010·Chemical Communications : Chem Comm·James L CarrAlan C Spivey
Feb 13, 2016·Annual Review of Biomedical Engineering·Hang Yin, Aaron D Flynn
May 7, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I Sayers, B A Helm
Feb 24, 2007·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Christine G DiRienzoRobert Korngold
Jul 31, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L RihakovaS Chemtob
May 1, 1999·The Journal of Biological Chemistry·M D HulettP M Hogarth
Mar 17, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Constance AuvynetChristophe Combadière
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bing-Hung ChenDaniel H Conrad
May 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christiane QuiniouSylvain Chemtob

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