PMID: 8587108Dec 1, 1995Paper

Structure-based sequence alignment of elongation factors Tu and G with related GTPases involved in translation

Journal of Molecular Evolution
A Avarsson

Abstract

The G domain and domain II in the crystal structure of Thermus thermophilus elongation factor G (EF-G) were compared with the homologous domains in Thermus aquaticus elongation factor Tu (EF-Tu). Sequence alignment derived from the structural superposition was used to define conserved sequence elements in domain II. These elements and previously known conserved sequence elements in the G domain were used to guide the alignment of the sequences of Sulfolobus acidocaldarius elongation factor 2, human elongation factor 2, and Escherichia coli initiation factor 2 and release factor 3 to the aligned sequences of EF-G and EF-Tu. This alignment, which deviates from previously published alignments, has evolutionary implications and leads to alternative interpretations of biochemical data concerning the interaction of elongation factors with the alpha-sarcin/ricin region of the ribosome. A single conserved sequence motif in domain II was identified and used to further characterize the GTPase subfamily of translation factors and related proteins. It was shown that the motif is found in most if not all the members of the family. Apparently, the common characteristic of these GTPases is an extensive consensus structural unit that possibly ...Continue Reading

Citations

Dec 1, 1995·Current Opinion in Structural Biology·A Liljas, M Garber
Aug 26, 2000·Progress in Biophysics and Molecular Biology·S Al-KaradaghiA Liljas
Jun 17, 1999·FEMS Microbiology Reviews·M V RodninaW Wintermeyer
Feb 17, 2000·The EMBO Journal·P NissenJ Nyborg
Jul 15, 2009·Tuberculosis·Anuradha SeshadriUmesh Varshney
Nov 8, 2007·Chemical Biology & Drug Design·Stefania StingoMaurizio Bifulco
Aug 25, 2015·Journal of Molecular Biology·Clayton SmithVitold E Galkin
Jan 28, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Myoung Seok KoJeong Woo Park
Oct 19, 2004·Trends in Immunology·John D MacMicking
Oct 31, 2000·Journal of Molecular Biology·M LaurbergA Liljas
Apr 18, 2013·Nature Communications·Göran WallinJohan Aqvist
Jul 25, 1998·FEBS Letters·J Nyborg, A Liljas

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