Structure Characterization and Otoprotective Effects of a New Endophytic Exopolysaccharide from Saffron

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Juan LiLu Wen

Abstract

Saffron, a kind of rare medicinal herb with antioxidant, antitumor, and anti-inflammatory activities, is the dry stigma of Crocus sativus L. A new water-soluble endophytic exopolysaccharide (EPS-2) was isolated from saffron by anion exchange chromatography and gel filtration. The chemical structure was characterized by FT-IR, GC-MS, and 1D and 2D-NMR spectra, indicating that EPS-2 has a main backbone of (1→2)-linked α-d-Manp, (1→2, 4)-linked α-d-Manp, (1→4)-linked α-d-Xylp, (1→2, 3, 5)-linked β-d-Araf, (1→6)- linked α-d-Glcp with α-d-Glcp-(1→ and α-d-Galp-(1→ as sidegroups. Furthermore, EPS-2 significantly attenuated gentamicin-induced cell damage in cultured HEI-OC1 cells and increased cell survival in zebrafish model. The results suggested that EPS-2 could protect cochlear hair cells from ototoxicity exposure. This study could provide new insights for studies on the pharmacological mechanisms of endophytic exopolysaccharides from saffron as otoprotective agents.

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Citations

Aug 21, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiangru FengJianxun Ding
Aug 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan RenAntonio Del Rio Flores

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Methods Mentioned

BETA
NMR
gel
acetylation

Software Mentioned

SPSS
Agilent

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