PMID: 11906609Mar 22, 2002Paper

Structure characterization of human serum proteins in solution and dry state

The Journal of Peptide Research : Official Journal of the American Peptide Society
S GorinsteinS Trakhtenberg

Abstract

The present report describes application of advanced analytical methods to establish correlation between changes in human serum proteins of patients with coronary atherosclerosis (protein metabolism) before and after moderate beer consumption. Intrinsic fluorescence, circular dichroism (CD), differential scanning calorimetry and hydrophobicity (So) were used to study human serum proteins. Globulin and albumin from human serum (HSG and HSA, respectively) were denatured with 8 m urea as the maximal concentration. The results obtained provided evidence of differences in their secondary and tertiary structures. The thermal denaturation of HSA and HSG expressed in temperature of denaturation (Td, degrees C), enthalpy (DeltaH, kcal/mol) and entropy (DeltaS kcal/mol K) showed qualitative changes in these protein fractions, which were characterized and compared with fluorescence and CD. Number of hydrogen bonds (n) ruptured during this process was calculated from these thermodynamic parameters and then used for determination of the degree of denaturation (%D). Unfolding of HSA and HSG fractions is a result of promoted interactions between exposed functional groups, which involve conformational changes of alpha-helix, beta-sheet and ape...Continue Reading

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Citations

Aug 21, 2013·International Journal of Biological Macromolecules·Nitin K PandeySwagata Dasgupta
Dec 24, 2003·The Journal of Nutritional Biochemistry·Shela GorinsteinSimon Trakhtenberg
Jan 5, 2016·Biological & Pharmaceutical Bulletin·Teppei TakahashiSeiichi Era
Feb 6, 2009·Biotechnology Progress·Iryna B SorokulovaVitaly Vodyanoy
Apr 20, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Shan-Yang LinShun-Li Wang
Nov 14, 2015·Vector Borne and Zoonotic Diseases·Yan-Jang S HuangDana L Vanlandingham
Jul 23, 2013·Journal of Biomolecular Structure & Dynamics·Nitin Kumar PandeySwagata Dasgupta
Apr 12, 2016·The Journal of Physical Chemistry. B·Maria Victoria NeacsuGabriela Ionita
Jun 18, 2016·Journal of Photochemistry and Photobiology. B, Biology·Gajalakshmi SekarNatarajan Chandrasekaran
Nov 1, 2012·Journal of Photochemistry and Photobiology. B, Biology·Daojin LiBaoming Ji
Jul 12, 2005·Journal of Photochemistry and Photobiology. B, Biology·Yan-Jun HuSong-Sheng Qu
Jan 3, 2009·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hua-Xin ZhangXi-Xiong Yang
Aug 2, 2017·Journal of Biomolecular Structure & Dynamics·Asma Yasmeen Khan, Gopinatha Suresh Kumar
Sep 10, 2004·Behavior Research Methods, Instruments, & Computers : a Journal of the Psychonomic Society, Inc·Arthur C GraesserMax M Louwerse
Oct 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Raja Mohamed Beema ShafreenShela Gorinstein
Jul 4, 2009·The Journal of Physical Chemistry. B·Josué JuárezVíctor Mosquera
Jan 23, 2009·The Journal of Physical Chemistry. B·Juan SabínFélix Sarmiento
Aug 6, 2010·The Journal of Physical Chemistry. B·Nitin K PandeySwagata Dasgupta
Jan 23, 2003·Journal of Agricultural and Food Chemistry·Shela GorinsteinSimon Trakhtenberg
Sep 24, 2014·The Journal of Physical Chemistry. B·Shubhatam SenSunando DasGupta

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