Structure-Dependent Binding of hnRNPA1 to Telomere RNA

Journal of the American Chemical Society
Xiao LiuYan Xu

Abstract

Telomeric repeat-containing RNA is a new noncoding RNA molecule that performs various biofunctions. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an RNA-binding protein involved in the telomere maintenance machinery. To date, little is known about how hnRNPA1 binds to telomeric RNA. In this study, we investigated the binding affinity and recognition mechanism of telomere RNA with the RNA recognition motif of hnRNPA1. Using the photochemical cross-linking method, we showed that the telomere RNA G-quadruplex with loops is important in the interaction of telomere RNA with hnRNPA1. Using small-molecule probes, we directly visualized the complex formed by the telomere RNA G-quadruplex and hnRNPA1 in vitro and in live cells. The results suggested that the structure-dependent binding of hnRNPA1 to telomere RNA regulates the telomere function. Therefore, our study provides new insights into the interactions between the RNA G-quadruplex and proteins at the telomere.

References

Jun 25, 1992·Nucleic Acids Research·M K ShoweL C Showe
Apr 11, 2006·RNA·Qing-Shuo ZhangAdrian R Krainer
Nov 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ryu TashiroHiroshi Sugiyama
Apr 3, 2007·Nature Protocols·Yan XuHiroshi Sugiyama
Dec 25, 2007·Nature Cell Biology·Stefan Schoeftner, Maria A Blasco
Jul 23, 2008·Journal of the American Chemical Society·Yan XuMakoto Komiyama
Feb 3, 2009·Journal of the American Chemical Society·Herry Martadinata, Anh Tuân Phan
Jun 17, 2009·Molecular and Cellular Biology·Corrado CasliniJay L Hess
Sep 19, 2009·The EMBO Journal·Jay SarthyPeter Baumann
Dec 22, 2009·Chemical Communications : Chem Comm·Gavin CollieStephen Neidle
Apr 24, 2010·Nucleic Acids Research·Gavin W CollieGary N Parkinson
May 13, 2010·Journal of the American Chemical Society·Yan XuMakoto Komiyama
Jun 23, 2010·Journal of the American Chemical Society·Gavin W CollieValérie Gabelica
Jul 27, 2010·FEBS Letters·Sascha FeuerhahnJoachim Lingner
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yan XuMakoto Komiyama
Oct 27, 2010·Nature Communications·Isabel López de SilanesMaria A Blasco
Dec 18, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ren Xu, Jian-Hua Mao
Jan 20, 2011·The FEBS Journal·Kentaro TakahamaTakanori Oyoshi
Mar 2, 2012·Current Pharmaceutical Design·Yan Xu
Jul 12, 2012·Journal of the American Chemical Society·Giulia BiffiShankar Balasubramanian
Mar 26, 2013·Chemistry & Biology·Kentaro TakahamaTakanori Oyoshi
Aug 6, 2013·Journal of the American Chemical Society·Wan Jun ChungAnh Tuân Phan
Aug 13, 2013·Nucleic Acids Research·Sophie RedonJoachim Lingner
Sep 10, 2013·Nature Structural & Molecular Biology·Bettina BalkBrian Luke
Dec 19, 2013·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Keisuke Iida, Kazuo Nagasawa
Sep 27, 2014·Trends in Cell Biology·Claus M Azzalin, Joachim Lingner
Nov 2, 2014·Nature Communications·Antonio PorroJoachim Lingner
Feb 14, 2015·International Journal of Biological Sciences·Caiqin WangShiming Lu
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Zhuo WangPaul M Lieberman
Nov 20, 2015·Nature Structural & Molecular Biology·Karsten Rippe, Brian Luke
Nov 8, 2016·Nature Chemical Biology·Carika WeldonCyril Dominguez

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Citations

Jan 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao Liu, Yan Xu
Apr 4, 2018·Chemical Communications : Chem Comm·Chao-Da XiaoYan Xu
Jul 28, 2018·Chemistry : a European Journal·Sefan AsamitsuHiroshi Sugiyama
Jan 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhi-Yin SunTian-Miao Ou
Feb 13, 2019·International Journal of Molecular Sciences·Sergey S ShishkinKsenia Lisitskaya
Feb 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antara SenguptaShantanu Chowdhury
Dec 5, 2019·Nucleic Acids Research·Ruby J RoachTracy K Hale
Nov 28, 2017·Organic & Biomolecular Chemistry·Zi-Qi LiChun-Qiong Zhou
Mar 5, 2020·Nucleic Acids Research·Meenakshi Ghosh, Mahavir Singh
Apr 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guanhui WuDanzhou Yang
Sep 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chao-Da XiaoYan Xu
Mar 20, 2020·Biochemistry·Abhijit SahaMarie-Paule Teulade-Fichou
Jul 20, 2018·ACS Omega·Takumi IshizukaYan Xu
Oct 12, 2021·Frontiers in Neuroscience·Myriam GagnéChristine Vande Velde

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