Structure determination of the exopolysaccharide produced by Lactobacillus rhamnosus strains RW-9595M and R

The Biochemical Journal
Marie-Rose Van CalsterenD Roy

Abstract

Exopolysaccharides (EPSs) were isolated and purified from Lactobacillus rhamnosus strains RW-9595M, which has been shown to possess cytokine-stimulating activity, and R grown under various fermentation conditions (carbon source, incubation temperature and duration). Identical (1)H NMR spectra were obtained in all cases. Molecular masses were determined by gel permeation chromatography. The primary structure was elucidated using chemical and spectroscopic techniques. Organic acid, monosaccharide and absolute configuration analyses gave the following composition: pyruvate, 1; D-glucose, 2; D-galactose, 1; and l-rhamnose, 4. Methylation analysis indicated the presence of three residues of 3-linked rhamnose, and one residue each of 2,3-linked rhamnose, 2-linked glucose, 3-linked glucose and 4,6-linked galactose. The EPS was submitted to periodate oxidation followed by borohydride reduction. Monosaccharide analysis of the resulting polysaccharide gave the new composition: rhamnose, 4; and glucose, 1. Methylation analysis confirmed the loss of the 2-linked glucose and 4,6-linked galactose residues. On the basis of one- and two-dimensional (1)H and (13)C NMR data, the structure of the native EPS was consistent with the following hepta...Continue Reading

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Citations

Jan 22, 2005·Applied Microbiology and Biotechnology·M I TorinoG Font de Valdez
Sep 24, 2005·Journal of Biomedical Optics·Shira L BroschatEdward Kuhn
Feb 13, 2015·Critical Reviews in Food Science and Nutrition·Nuria SalazarPatricia Ruas-Madiedo
Jan 1, 2004·The Journal of Biological Chemistry·Yann GuerardelGradimir N Misevic
Dec 1, 2012·Probiotics and Antimicrobial Proteins·Claudio Hidalgo-CantabranaPatricia Ruas-Madiedo

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