Structure Determination of the Nuclear Pore Complex with Three-Dimensional Cryo electron Microscopy

Journal of Molecular Biology
Alexander von Appen, Martin Beck

Abstract

Determining the structure of the nuclear pore complex (NPC) imposes an enormous challenge due to its size, intricate composition and membrane-embedded nature. In vertebrates, about 1000 protein building blocks assemble into a 110-MDa complex that fuses the inner and outer membranes of a cell's nucleus. Here, we review the recent progress in understanding the in situ architecture of the NPC with a specific focus on approaches using three-dimensional cryo electron microscopy. We discuss technological benefits and limitations and give an outlook toward obtaining a high-resolution structure of the NPC.

References

Jun 26, 1992·Cell·J E HinshawR A Milligan
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Apr 1, 1982·The Journal of Cell Biology·P N Unwin, R A Milligan
Aug 1, 1982·Journal of Microscopy·W O Saxton, W Baumeister
Apr 28, 1995·Journal of Molecular Biology·C W Akey
Nov 1, 1993·The Journal of Cell Biology·M P Rout, G Blobel
Sep 3, 1999·The Journal of Biological Chemistry·N R Yaseen, G Blobel
Feb 23, 2000·The Journal of Cell Biology·M P RoutB T Chait
May 10, 2000·The Journal of Biological Chemistry·S M BailerE Hurt
Feb 21, 2002·Molecular Biology of the Cell·Nelly Panté, Michael Kann
Oct 13, 1950·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H G CALLAN, S G TOMLIN
May 18, 2004·Molecular Biology of the Cell·Isabelle LoïodiceValérie Doye
Mar 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Friedrich FörsterDeborah Fass
Apr 20, 2005·Current Opinion in Structural Biology·Thomas U Schwartz
Jun 20, 2006·Nature Cell Biology·Abel R Alcázar-RománSusan R Wente
Nov 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Beth A RasalaDouglass J Forbes
Jun 9, 2009·Nature Structural & Molecular Biology·Martin Kampmann, Günter Blobel
Jun 23, 2009·EMBO Reports·Maya Capelson, Martin W Hetzer
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hyuk-Soo SeoAndré Hoelz
Apr 14, 2010·Molecular Cell·Alwin Köhler, Ed Hurt
Oct 27, 2010·The Journal of Cell Biology·Jana M MitchellRichard W Wozniak
Apr 19, 2011·Annual Review of Biochemistry·André HoelzGünter Blobel
Apr 3, 2012·Molecular Cell·Andreas WernerFrauke Melchior
May 29, 2012·Structure·Tal MaimonOhad Medalia
Jul 31, 2012·Nature Methods·Sjors H W Scheres, Shaoxia Chen
Mar 8, 2013·Current Opinion in Structural Biology·John A G Briggs
Dec 24, 2013·Journal of Cell Science·Nathalie EisenhardtWolfram Antonin

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Citations

Jan 23, 2016·Journal of Molecular Biology·Edward A Lemke
Apr 10, 2016·Journal of Molecular Biology·Dirk Görlich, Richard W Kriwacki
Sep 8, 2016·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Hicham Saad, Jennifer A Cobb
Dec 22, 2016·Nature Reviews. Molecular Cell Biology·Martin Beck, Ed Hurt
Jan 25, 2017·Viruses·Lara Gallucci, Michael Kann
Mar 3, 2017·PLoS Pathogens·Michael P RoutMark C Field
Sep 10, 2017·The Journal of General Physiology·Joan PulupaSanford M Simon
Feb 17, 2017·Journal of Cell Science·Jiong MaWeidong Yang
Nov 6, 2018·Canadian Journal of Physiology and Pharmacology·Claudia C PrestonRandolph S Faustino
Apr 4, 2019·Annual Review of Biophysics·Bernhard HampoelzMartin Beck
May 16, 2019·Biological Chemistry·Fotis L KyrilisPanagiotis L Kastritis
Sep 1, 2018·Epigenomes·Claudia C Preston, Randolph S Faustino
Aug 15, 2019·The Protein Journal·Joseph S Glavy
Mar 15, 2018·Nature·Seung Joong KimMichael P Rout
Mar 19, 2019·Annual Review of Biochemistry·Daniel H Lin, André Hoelz
Oct 2, 2019·Cells·Ekta Shukla, Radha Chauhan
Dec 17, 2019·Biophysical Journal·Kai HuangIgal Szleifer
Jan 12, 2021·Computational and Structural Biotechnology Journal·Samantha J ZieglerYannick J Bomble

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Methods Mentioned

BETA
electron microscopy
X-ray
electron tomography
superresolution microscopy
light microscopy

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