Structure, expression, and extracellular targeting of PM27, a skeletal protein associated specifically with growth of the sea urchin larval spicule

Developmental Biology
M A HarkeyR A Raff

Abstract

The PM27 gene encodes a sea urchin skeletal protein. Both the transcript and encoded protein appear at the mesenchyme blastula stage and are restricted to the primary mesenchyme cell (PMC) lineage throughout development. Transgenic expression of PM27 promoter constructs demonstrates that this cell specificity is regulated at the level of transcription. The PM27 sequence predicts a nonglycosylated secretory product of 27 kDa in mature form. The N-terminal "repeat" domain of the deduced amino acid sequence consists of a series of tandem repeats and shares both sequence and predicted structural similarities with several fiber-forming proteins. The C-terminal "lectin" domain is similar to the C-class lectins. Antisera against either domain of PM27 detect two major proteins in embryo extracts, with apparent molecular weights of 27 and 30 kDa. Immunolocalization in whole embryos demonstrates that PM27 antigen is produced uniformly and exclusively by PMCs through the early prism stage and that this specificity is further restricted during skeletogenesis to a subpopulation of PMCs associated with the growing tips of the spicules. It is secreted to the skeletal compartment and accumulates predominantly at the advancing mineralizing surf...Continue Reading

Citations

Aug 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F H Wilt
Sep 27, 2003·Developmental Biology·Vincenzo CavalieriMaria Di Bernardo
Sep 2, 2000·Development, Growth & Differentiation·T Kitajima, H Urakami
Aug 13, 2008·Proteome Science·Karlheinz MannMatthias Mann
Jun 23, 2010·Proteome Science·Karlheinz MannAlbert J Poustka
Jul 20, 2002·Zoological Science·Fred H Wilt
Jun 10, 2010·Zoological Science·Yuka OkamitsuNaoaki Sakamoto
May 31, 2011·Frontiers in Bioscience (Landmark Edition)·Arthur Veis
Jan 18, 2007·Evolution & Development·Alan C LoveRudolf A Raff
Jun 26, 2003·Differentiation; Research in Biological Diversity·Fred H WiltBrian T Livingston
Sep 15, 2004·Journal of Structural Biology·Jong SetoFred Wilt
Apr 17, 2014·Zygote : the Biology of Gametes and Early Embryos·Nancy M Mozingo
May 25, 2002·Developmental Biology·Eric H DavidsonHamid Bolouri
Sep 4, 1999·Journal of Structural Biology·F H Wilt
Dec 3, 2014·Gene Expression Patterns : GEP·Zhongling Sun, Charles A Ettensohn
Jun 5, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Margaret S TellisChris M Wood
Mar 3, 2011·Developmental Biology·Ashrifia Adomako-Ankomah, Charles A Ettensohn
Aug 26, 2004·Developmental Biology·Ochan OtimEric H Davidson
Jun 7, 2005·Developmental Biology·Melani S Cheers, Charles A Ettensohn
Jan 27, 2015·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Julia M KanoldFrédéric Marin
Aug 17, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L AmeyeP Dubois
Dec 3, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Eric P IngersollFred H Wilt
Sep 29, 2018·Genesis : the Journal of Genetics and Development·Tanvi ShashikantCharles A Ettensohn
Mar 16, 2007·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Amanda L Rychel, Billie J Swalla
Sep 30, 2008·Chemical Reviews·Christopher E Killian, Fred H Wilt
Dec 23, 2011·Development·Kiran RafiqCharles A Ettensohn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.