Structure, Expression, and Functional Analysis of the Hexokinase Gene Family in Cassava

International Journal of Molecular Sciences
Meng-Ting GengJian-Chun Guo

Abstract

Hexokinase (HXK) proteins play important roles in catalyzing hexose phosphorylation and sugar sensing and signaling. To investigate the roles of HXKs in cassava tuber root development, seven HXK genes (MeHXK1-7) were isolated and analyzed. A phylogenetic analysis revealed that the MeHXK family can be divided into five subfamilies of plant HXKs. MeHXKs were clearly divided into type A (MeHXK1) and type B (MeHXK2-7) based on their N-terminal sequences. MeHXK1-5 all had typical conserved regions and similar protein structures to the HXKs of other plants; while MeHXK6-7 lacked some of the conserved regions. An expression analysis of the MeHXK genes in cassava organs or tissues demonstrated that MeHXK2 is the dominant HXK in all the examined tissues (leaves, stems, fruits, tuber phloems, and tuber xylems). Notably, the expression of MeHXK2 and the enzymatic activity of HXK were higher at the initial and expanding tuber stages, and lower at the mature tuber stage. Furthermore, the HXK activity of MeHXK2 was identified by functional complementation of the HXK-deficient yeast strain YSH7.4-3C (hxk1, hxk2, glk1). The gene expression and enzymatic activity of MeHXK2 suggest that it might be the main enzyme for hexose phosphorylation duri...Continue Reading

References

Feb 24, 2001·Genome Biology·T Richmond
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Oct 17, 2008·Phytochemistry·David Granot
Aug 27, 2009·Journal of Experimental Botany·Abhijit Karve, Brandon D Moore
Feb 16, 2011·BMC Plant Biology·Anders NilssonHans Ronne
Mar 2, 2011·Molecular Plant Pathology·Johannes SiemensJutta Ludwig-Müller
Apr 24, 2012·Tropical Plant Biology·Simon ProchnikSteve Rounsley
Dec 19, 2012·Protoplasma·Ashwini GodboleMathew K Mathew
Dec 26, 2012·Plant Science : an International Journal of Experimental Plant Biology·Rakefet David-SchwartzDavid Granot
Jan 12, 2013·Plant, Cell & Environment·Young-Min KimMohammad-Reza Hajirezaei
Mar 15, 2013·Frontiers in Plant Science·David GranotGilor Kelly
Dec 3, 2013·Journal of Experimental Botany·David GranotRakefet David-Schwartz
Apr 11, 2014·Journal of Experimental Botany·Manjul SinghAshverya Laxmi
Dec 23, 2014·Journal of Plant Biology = Singmul Hakhoe Chi·Jen Sheen
May 8, 2015·Journal of Integrative Plant Biology·Hyun-Bi KimJong-Seong Jeon
Feb 20, 2016·Scientific Reports·You-Zhi LiBao-Shan Chen
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Jun 1, 2007·Functional Plant Biology : FPB·David Granot

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Methods Mentioned

BETA
reverse transcription PCR
PCR
protein assay

Software Mentioned

DNAman
MEGA7
QMEAN
SwissModel
Muscle
BLAST
PyMOL
Gene Structure Display

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