PMID: 9655503Jul 9, 1998Paper

Structure-function analysis of cell adhesion by neural (N-) cadherin

Neuron
K TamuraL Shapiro

Abstract

To investigate the possible biological function of the lateral "strand dimer" observed in crystal structures of a D1 domain extracellular fragment from N-cadherin, we have undertaken site-directed mutagenesis studies of this molecule. Mutation of most residues important in the strand dimer interface abolish the ability of N-cadherin to mediate cell adhesion. Mutation of an analogous central residue (Trp-2) in E-cadherin also abrogates the adhesive capacity of that molecule. We also determined the crystal structure of a Ca2+-complexed two-domain fragment from N-cadherin. This structure, like its E-cadherin counterpart, does not adopt the strand dimer conformation. This suggests the possibility that classical cadherins might stably exist in both dimeric and monomeric forms. Data from several laboratories imply that lateral dimerization or clustering of cadherins may increase their adhesivity. We suggest the possibility that the strand dimer may play a role in this activation.

References

Jun 1, 1992·Seminars in Cell Biology·R Kemler
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·M TakeichiA Nagafuchi
Jan 1, 1990·Annual Review of Biochemistry·M Takeichi
Oct 1, 1990·The Journal of Cell Biology·M Ozawa, R Kemler
Apr 1, 1990·The Journal of Cell Biology·J L Bixby, R Zhang
May 1, 1990·Developmental Biology·O W BlaschukY Pouliot
Aug 11, 1989·Science·S MiyataniM Takeichi
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·L ShapiroW A Hendrickson
Mar 23, 1995·Nature·L ShapiroW A Hendrickson
Oct 1, 1996·The Journal of Cell Biology·W M BrieherB M Gumbiner
Nov 1, 1996·Cell Adhesion and Communication·D HaneinB Geiger
Jan 1, 1997·Molecular and Cellular Neurosciences·D R Colman

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Citations

May 20, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K Vleminckx, R Kemler
May 9, 2000·Biochemical and Biophysical Research Communications·M KitagawaS T Suzuki
Jan 28, 2012·Cell and Tissue Research·Orest W Blaschuk
Dec 17, 2003·Current Opinion in Structural Biology·Saurabh D PatelLawrence Shapiro
Oct 31, 2003·Trends in Neurosciences·Francisco G Scholl, Peter Scheiffele
Jan 1, 2003·Journal of Molecular Biology·Thomas AhrensJürgen Engel
Apr 25, 2000·Progress in Neurobiology·C Redies
Jan 13, 2000·Biophysical Chemistry·W S ShanL Shapiro
Sep 9, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B Ranscht
Sep 19, 2003·Genomics·George KirovMichael J Owen
Sep 9, 2000·Current Opinion in Cell Biology·D Leckband, S Sivasankar
Sep 9, 2000·Current Opinion in Cell Biology·J L Brusés
Sep 9, 2000·Current Opinion in Cell Biology·H Kamiguchi, V Lemmon
Oct 6, 1999·Current Opinion in Cell Biology·A KusumiK Koyasako
Oct 6, 1999·Current Opinion in Cell Biology·S M Troyanovsky
Nov 13, 1998·Current Opinion in Neurobiology·L Shapiro, D R Colman
May 14, 1999·Current Opinion in Structural Biology·A W KochJ Engel
Oct 26, 2000·Trends in Cell Biology·D L BensonG W Huntley
Aug 30, 2011·Biochemistry·Nagamani Vunnam, Susan Pedigo
Mar 22, 2000·The Journal of Peptide Research : Official Journal of the American Peptide Society·W YangJ J Yang
Apr 20, 2005·Nature Genetics·Shalini JadejaPeter J Scambler
Mar 2, 2010·Nature Structural & Molecular Biology·Carlo CiattoLawrence Shapiro
Mar 2, 2010·Nature Structural & Molecular Biology·Oliver J HarrisonLawrence Shapiro
May 17, 2011·Nature Structural & Molecular Biology·Jeremie VendomeBarry Honig
Feb 21, 2002·The Biochemical Journal·Shabih-e-Hassnain SyedAnthony I Magee
Oct 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·A K PrakasamD E Leckband
Dec 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yunxiang ZhangSteven Chu
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Heather M ElledgeUlrich Müller
May 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sally A KimErin M Schuman
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SivasankarD Leckband
Jun 17, 2008·The Journal of Biological Chemistry·Quanming ShiDeborah Leckband
Oct 5, 2012·Journal of Biomolecular Structure & Dynamics·Ruchuan LiuJean Paul Thiery
May 3, 2000·The Journal of Experimental Medicine·K S TaraszkaM B Brenner
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Lawrence Shapiro, William I Weis

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