Structure-function analysis of photosystem II subunit S (PsbS) in vivo

Functional Plant Biology : FPB
Xiao-Ping LiKrishna K Niyogi

Abstract

In land plants, photosystem II subunit S (PsbS) plays a key role in xanthophyll- and pH-dependent non-photochemical quenching (qE) of excess absorbed light energy. Arabidopsis thaliana (L.) Heynh. npq4 mutants are defective in the psbS gene and have impaired qE. Exactly how the PsbS protein is involved in qE is unclear, but it has been proposed that PsbS binds H+ and/or de-epoxidized xanthophylls in excess light as part of the qE mechanism. To identify amino acid residues that are important for PsbS function, we sequenced the psbS gene from eight npq4 point mutant alleles isolated by forward genetics screening, including two new alleles. In the four transmembrane helices of PsbS, several amino acid residues were found to affect the stability and/or function of the protein. By comparing the predicted amino acid sequences of PsbS from several plant species and studying the proposed topological structure of PsbS, eight possible H+-binding amino acid residues on the lumenal side of the protein were identified and then altered by site-directed mutagenesis in vitro. The mutant psbS genes were transformed into npq4-1, a psbS deletion mutant, to test the stability and function of the mutant PsbS proteins invivo. The results demonstrate...Continue Reading

Citations

Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Ping LiKrishna K Niyogi
Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ying-Zhong MaGraham R Fleming
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elisabetta BergantinoIldikò Szabò
Sep 10, 2005·Photosynthesis Research·Alexander RubanAnne-Lise Etienne
Apr 20, 2006·Photosynthesis Research·Barry A LoganA Scott Holaday
Sep 2, 2006·The New Phytologist·Barbara Demmig-Adams, William W Adams
Dec 12, 2007·The Journal of Biological Chemistry·Giulia BonenteRoberto Bassi
Dec 7, 2007·The Journal of Biological Chemistry·Anett Z KissPeter Horton
Mar 29, 2011·Annual Review of Plant Biology·Martin F Hohmann-Marriott, Robert E Blankenship
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Laura WilkWerner Kühlbrandt
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Matthew D BrooksKrishna K Niyogi
Aug 11, 2015·Nature Structural & Molecular Biology·Minrui FanWenrui Chang
Mar 23, 2004·The Journal of Biological Chemistry·Xiao-Ping LiKrishna K Niyogi
Mar 21, 2006·FEBS Letters·Sophie CrouchmanPeter Horton
Mar 23, 2006·Journal of Experimental Botany·Ulrich HeberVladimir A Shuvalov
Jul 7, 2009·Annual Review of Plant Biology·Zhirong LiKrishna K Niyogi
Feb 22, 2011·Plant, Cell & Environment·Caterina GerottoTomas Morosinotto
Dec 7, 2017·Plant, Cell & Environment·Alexandra J TownsendAlexander V Ruban
Apr 17, 2020·Photosynthesis Research·Nicoletta LiguoriSebastian Thallmair
Mar 25, 2009·The Journal of Biological Chemistry·Nico BetterleRoberto Bassi
Jun 25, 2013·Photosynthesis Research·Julia ZaksGraham R Fleming
Aug 14, 2019·Photochemistry and Photobiology·Brian Ospina Calvo, María Gabriela Lagorio
Sep 1, 2007·Functional Plant Biology : FPB·Jose I García-PlazaolaC Barry Osmond
Dec 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mark Aspinall-O'DeaPeter Horton
Jul 1, 2008·Plant & Cell Physiology·Irina GrounevaReimund Goss
May 1, 2018·Plant, Cell & Environment·Diep R GangulyBarry J Pogson

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