Structure-Function Analysis of the Curli Accessory Protein CsgE Defines Surfaces Essential for Coordinating Amyloid Fiber Formation.

MBio
Roger D KleinMatthew R Chapman

Abstract

Curli amyloid fibers are produced as part of the extracellular biofilm matrix and are composed primarily of the major structural subunit CsgA. The CsgE chaperone facilitates the secretion of CsgA through CsgG by forming a cap at the base of the nonameric CsgG outer membrane pore. We elucidated a series of finely tuned nonpolar and charge-charge interactions that facilitate the oligomerization of CsgE and its ability to transport unfolded CsgA to CsgG for translocation. CsgE oligomerization in vitro is temperature dependent and is disrupted by mutations in the W48 and F79 residues. Using nuclear magnetic resonance (NMR), we identified two regions of CsgE involved in the CsgE-CsgA interaction: a head comprising a positively charged patch centered around R47 and a stem comprising a negatively charged patch containing E31 and E85. Negatively charged residues in the intrinsically disordered N- and C-terminal "tails" were not implicated in this interaction. Head and stem residues were mutated and interrogated using in vivo measurements of curli production and in vitro amyloid polymerization assays. The R47 head residue of CsgE is required for stabilization of CsgA- and CsgE-mediated curli fiber formation. Mutation of the E31 and E85 ...Continue Reading

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Citations

Feb 20, 2020·Nature Reviews. Microbiology·Roger D Klein, Scott J Hultgren
Jan 15, 2020·Nature Communications·Zhaofeng YanXueming Li
Nov 29, 2020·Microbial Biotechnology·Dongru ChenHuancai Lin
May 1, 2021·Pathogens·Khushal KhambhatiNeha Jain
May 11, 2021·Frontiers in Cellular and Infection Microbiology·Yiying HuangLing Long
Jun 8, 2019·Microbiology Spectrum·Kevin O TamadonfarScott J Hultgren
Aug 28, 2021·Biomedicines·Dan Cristian GheorgheAlexandru Mihai Grumezescu
Aug 4, 2021·Annual Review of Microbiology·Diego O Serra, Regine Hengge

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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
size exclusion chromatography
size
light scattering
protein folding
static light scattering

Software Mentioned

Ensembl
Pal2Nal webserver
NMRFAM
- SPARKY
DataMonkey webserver
MAFFT
Quantity One
Geneious R10

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