Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

Alexandra C WallsDavid Veesler


The emergence of SARS-CoV-2 has resulted in >90,000 infections and >3,000 deaths. Coronavirus spike (S) glycoproteins promote entry into cells and are the main target of antibodies. We show that SARS-CoV-2 S uses ACE2 to enter cells and that the receptor-binding domains of SARS-CoV-2 S and SARS-CoV S bind with similar affinities to human ACE2, correlating with the efficient spread of SARS-CoV-2 among humans. We found that the SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs. We determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer, providing a blueprint for the design of vaccines and inhibitors of viral entry. Finally, we demonstrate that SARS-CoV S murine polyclonal antibodies potently inhibited SARS-CoV-2 S mediated entry into cells, indicating that cross-neutralizing antibodies targeting conserved S epitopes can be elicited upon vaccination.


Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R VlasakP Palese
Feb 1, 1994·Trends in Microbiology·H D Klenk, W Garten
Jul 29, 1998·Protein Engineering·K A MiroshnikovV V Mesyanzhinov
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Jan 31, 2004·Science·UNKNOWN Chinese SARS Molecular Epidemiology Consortium
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jianhua SuiWayne A Marasco
Mar 26, 2005·The EMBO Journal·Wenhui LiMichael Farzan
May 14, 2005·Journal of Structural Biology·Christian SulowayBridget Carragher
Oct 1, 2005·Science·Wendong LiLin-Fa Wang
Feb 21, 2006·Emerging Infectious Diseases·Ming WangJianguo Xu
Sep 7, 2006·The Journal of Biological Chemistry·William C HwangRobert C Liddington
Sep 12, 2006·Journal of Structural Biology·Thomas D GoddardThomas E Ferrin
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sandrine BelouzardGary R Whittaker
Nov 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kailang WuFang Li
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
May 17, 2012·Viruses·Taylor Heald-Sargent, Tom Gallagher
Jul 31, 2012·Nature Methods·Sjors H W Scheres, Shaoxia Chen
Oct 19, 2012·The New England Journal of Medicine·Ali M ZakiRon A M Fouchier
Nov 12, 2013·Emerging Infectious Diseases·Ziad A MemishW Ian Lipkin
Dec 21, 2013·The Lancet Infectious Diseases·Bart L HaagmansMarion P G Koopmans
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yang YangFang Li
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jean Kaoru Millet, Gary R Whittaker
Dec 3, 2014·Virus Research·Jean Kaoru Millet, Gary R Whittaker

❮ Previous
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Apr 16, 2020·Circulation·Peter P LiuHongliang Li
Apr 5, 2020·Journal of Clinical Medicine·Pietro H GuzziFederico M Giorgi
Mar 28, 2020·Journal of Gastroenterology and Hepatology·Sunny H WongJoseph Jy Sung
Apr 1, 2020·Nature·Jian ShangFang Li
Apr 25, 2020·JDR Clinical and Translational Research·Y F RenE Eliav
Apr 26, 2020·Microbial Biotechnology·Wei E HuangZhanfeng Cui
Apr 16, 2020·Journal of Medical Virology·Adam Brufsky
Apr 11, 2020·Physiological Genomics·Rachel M GolonkaMatam Vijay-Kumar
May 1, 2020·Journal of Cardiovascular Pharmacology and Therapeutics·Himanshu SankrityayanAnil Bhanudas Gaikwad
Apr 21, 2020·Journal of Biomolecular Structure & Dynamics·Subramanian BoopathiPonmalai Kolandaivel
May 2, 2020·The New England Journal of Medicine·Mandeep R MehraAmit N Patel
Apr 3, 2020·Vox Sanguinis·Pierre TiberghienYazdan Yazdanpanah
Mar 26, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zaid A AbassiZaher Armaly
Apr 23, 2020·Clinical Pharmacology and Therapeutics·Krishna Sriram, Paul A Insel
Apr 11, 2020·Basic Research in Cardiology·Paola RizzoRoberto Ferrari
May 13, 2020·Journal of the American Heart Association·Anthony S LarsonWaleed Brinjikji
May 14, 2020·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Robert C Penner
May 5, 2020·Journal of Biomolecular Structure & Dynamics·Naún Lobo-GaloÁngel Gabriel Díaz-Sánchez
Apr 2, 2020·ELife·Yinon M Bar-OnRon Milo
Mar 28, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas C HanffAmir M Mohareb
Apr 17, 2020·Journal of Thrombosis and Thrombolysis·Richard C Becker
Apr 26, 2020·British Journal of Pharmacology·Krishna Sriram, Paul A Insel
May 23, 2020·PLoS Pathogens·Daolin TangRui Kang
May 29, 2020·Journal of Biomolecular Structure & Dynamics·Subhomoi Borkotoky, Manidipa Banerjee
May 23, 2020·Diagnostics·Panagiota I KontouPantelis G Bagos
May 20, 2020·Journal of Clinical Medicine·Umesh KalathiyaJavier Antonio Alfaro
May 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elisa BarileMaurizio Pellecchia
May 30, 2020·Vector Borne and Zoonotic Diseases·Willy A Valdivia-Granda, Jürgen A Richt
Jun 2, 2020·Hypertension·Paolo VerdecchiaFabio Angeli
Apr 30, 2020·Nature Reviews. Immunology·Matthew Zirui TayLisa F P Ng
May 19, 2020·American Journal of Physiology. Endocrinology and Metabolism·Johnny S YounisKarl Skorecki
May 7, 2020·International Journal of Clinical Practice·Manuel Becerra-Flores, Timothy Cardozo
Jun 6, 2020·VASA. Zeitschrift für Gefässkrankheiten·Birgit LinnemannMichael Lichtenberg
May 3, 2020·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Paolo Maria MatricardiRoberto Nisini
Jun 9, 2020·Biochemical Society Transactions·Spencer J Williams, Ethan D Goddard-Borger

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Methods Mentioned

gel filtration
biolayer interferometry

Software Mentioned

UCSF Chimera

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