Structure-function correlation of tight junctional impairment after intrahepatic and extrahepatic cholestasis in rat liver

Gastroenterology
C RahnerL Landmann

Abstract

Tight junctions, the only barrier between blood and bile, are crucial in bile formation. The aim of this study was to correlate changes in morphology and permeability by comparing structural parameters with marker secretion into normal and cholestatic rat bile. Cholestasis was induced by bile duct ligation of 5 and 21 days of ethinylestradiol administration. Quantitated structural parameters induced junctional length, strand number, junctional depth, and spacing of junctional particles. Junctional permeability was probed with horseradish peroxidase and dextrans of increasing sizes. Junctional length was decreased slightly by ethinylestradiol (-16% after 21 days) but increased by ligation (77%). Mean strand number decreased from 4.6 to 3.7 after 21 days of ethinylestradiol and 3.4 after ligation associated with increased junctional depth. The proportions of morphologically horseradish peroxidase-positive junctions increased from 4% to 15% after 21 days of ethinylestradiol and to 56% after ligation. Horseradish peroxidase secretion was increased twofold by ethinylestradiol and 6.5-fold by ligation, paralleled by an increase of dextran size selectivity from 70,000 to 79,000 daltons after ethinylestradiol and to 266,000 daltons aft...Continue Reading

Citations

Oct 22, 1998·The New England Journal of Medicine·M TraunerJ L Boyer
Jun 27, 2008·Journal of Virology·Michelle J SnooksDavid A Anderson
Feb 20, 2010·Journal of Biomedicine & Biotechnology·Isabella Ellinger, Renate Fuchs
Mar 8, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C C PaulusmaR P Oude Elferink
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D RostD Keppler
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T KawaguchiK Tanikawa
Dec 13, 2005·Critical Care Medicine·Kevin W McConnell, Craig M Coopersmith
Oct 28, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Brigitte GrosseEmmanuel Gonzales
Oct 31, 2014·Scientific Reports·Keira Joann HerrJean Paul Thiery
Oct 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Janet M LarkinVu Tinh
Apr 2, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lin Wang, James L Boyer
Aug 23, 2008·Journal of Gastroenterology and Hepatology·Pavel TomsikStanislav Micuda
Aug 4, 2006·Microscopy Research and Technique·Permsin MarbetLukas Landmann
Aug 30, 2007·Journal of Gastroenterology and Hepatology·Hideyuki KojimaHiroshi Fukui
May 23, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Delphine FirrincieliNicolas Chignard
Oct 15, 2013·Biology of the Cell·Sylviane BoucherieLaurent Combettes
Oct 22, 2013·Molecular Aspects of Medicine·Marion J PollheimerBruno Stieger
Jan 12, 2011·Biochemical Pharmacology·Su-Young ChoiHyunyoung Jeong
Mar 26, 2014·Tissue Barriers·R K Rao, G Samak
Apr 20, 2016·Archives of Toxicology·Xiaojiaoyang LiLuyong Zhang
Oct 24, 1998·Biochimica Et Biophysica Acta·N R KoopenF Kuipers
Nov 24, 2016·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Janske ReilingJonathan Fawcett
Apr 29, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L LandmannB Stieger
Sep 27, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shi-Ying Cai, James L Boyer
Nov 11, 2008·Wiener medizinische Wochenschrift·Isabella Ellinger, Renate Fuchs
Nov 11, 2008·Wiener medizinische Wochenschrift·Wolfgang JessnerJürg Graf
Apr 22, 2003·Nature Genetics·Victoria E H CarltonLaura N Bull
Mar 8, 2020·Liver International : Official Journal of the International Association for the Study of the Liver·Grégory MerlenThierry Tordjmann
Aug 30, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiaonan HanRussell L Delude
Sep 25, 2019·International Journal of Molecular Sciences·Luis E Soria-JassoEduardo Fernández-Martínez
Jul 18, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·S M WienerB Safer
Mar 10, 2001·Clinics in Liver Disease·G A Kullak-Ublick, P J Meier
Jan 17, 2019·Gene Expression·Tirthadipa Pradhan-Sundd, Satdarshan Pal Monga

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.