PMID: 8955409Dec 1, 1996Paper

Structure-function relationships among wild-type variants of Staphylococcus aureus beta-lactamase: importance of amino acids 128 and 216

Journal of Bacteriology
R K VoladriD S Kernodle

Abstract

beta-Lactamases inactivate penicillin and cephalosporin antibiotics by hydrolysis of the beta-lactam ring and are an important mechanism of resistance for many bacterial pathogens. Four wild-type variants of Staphylococcus aureus beta-lactamase, designated A, B, C, and D, have been identified. Although distinguishable kinetically, they differ in primary structure by only a few amino acids. Using the reported sequences of the A, C, and D enzymes along with crystallographic data about the structure of the type A enzyme to identify amino acid differences located close to the active site, we hypothesized that these differences might explain the kinetic heterogeneity of the wild-type beta-lactamases. To test this hypothesis, genes encoding the type A, C, and D beta-lactamases were modified by site-directed mutagenesis, yielding mutant enzymes with single amino acid substitutions. The substitution of asparagine for serine at residue 216 of type A beta-lactamase resulted in a kinetic profile indistinguishable from that of type C beta-lactamase, whereas the substitution of serine for asparagine at the same site in the type C enzyme produced a kinetic type A mutant. Similar bidirectional substitutions identified the threonine-to-alanine...Continue Reading

References

Jan 5, 1979·Molecular & General Genetics : MGG·S Chang, S N Cohen
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 1, 1992·Antimicrobial Agents and Chemotherapy·D J ZygmuntD S Kernodle
Sep 1, 1991·Antimicrobial Agents and Chemotherapy·G A Jacoby, A A Medeiros
May 15, 1991·The Biochemical Journal·R P AmblerS G Waley
Jan 1, 1989·The Journal of Infectious Diseases·D S KernodleP A McGraw
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Nov 1, 1987·Antimicrobial Agents and Chemotherapy·D W GalettoG L Archer
Aug 5, 1987·Journal of Molecular Biology·S MillerC Chothia
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jul 1, 1971·Journal of Bacteriology·L H Johnston, K G Dyke
Jun 1, 1974·The Biochemical Journal·S G Waley
Dec 1, 1972·Journal of General Microbiology·R W Lacey, J Grinsted
Jul 1, 1973·Analytical Biochemistry·G W RossC H O'Callaghan
Jan 1, 1984·British Medical Bulletin·A A Medeiros
May 16, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R P Ambler
Jun 1, 1995·Antimicrobial Agents and Chemotherapy·K BushA A Medeiros

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Citations

Jul 8, 2014·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Shinwon LeeShin-Woo Kim
Feb 28, 2020·Clinical Microbiology Reviews·Karen Bush, Patricia A Bradford
Jan 15, 2016·Journal of Clinical Microbiology·S S RichterD J Diekema
Jan 27, 2004·Antimicrobial Agents and Chemotherapy·Geoffrey W StoneNafsika H Georgopapadakou
Feb 20, 2020·Antimicrobial Agents and Chemotherapy·Lina P CarvajalJinnethe Reyes
Jul 16, 2003·Biochemistry·Xiaojun WangBrian K Shoichet

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