PMID: 9006045Feb 1, 1997Paper

Structure-function studies of the adenylate cyclase toxin of Bordetella pertussis and the leukotoxin of Pasteurella haemolytica by heterologous C protein activation and construction of hybrid proteins

Journal of Bacteriology
G D WestropJ G Coote

Abstract

The adenylate cyclase toxin (CyaA) from Bordetella pertussis and the leukotoxin (LktA) from Pasteurella haemolytica are members of the RTX (stands for repeats in toxin) family of cytolytic toxins. They have pore-forming activity and share significant amino acid homology but show marked differences in biological activity. CyaA is an invasive adenylate cyclase and a weak hemolysin which is active on a wide range of mammalian cells. LktA is a cytolytic protein with a high target cell specificity and is able to lyse only leukocytes and platelets from ruminants. Each toxin is synthesized as an inactive protoxin encoded by the A gene, and the product of the accessory C gene is required for posttranslational activation. Heterologous activation of LktA by CyaC did not result in a change in its specificity for nucleated cells, although the toxin showed a greater hemolytic-to-cytotoxic ratio. LktC was unable to activate CyaA. A hybrid toxin (Hyb1), which contained the N-terminal enzymic domain and the pore-forming domain from CyaA (amino acids [aa] 1 to 687), with the remainder of the protein derived from the C-terminal end of LktA (aa 379 to 953), showed no toxic activity. Replacement of part of the LktA C-terminal domain of Hyb1 by the...Continue Reading

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Citations

Jul 20, 2010·Molecular Genetics and Genomics : MGG·Yusuke YoshidaKozo Makino
Apr 1, 2003·Animal Health Research Reviews·Samithamby JeyaseelanSamuel K Maheswaran
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Annika Röttig, Alexander Steinbüchel
Jul 11, 1998·The Journal of Biological Chemistry·M GrayE Hewlett
Jun 10, 2010·FEMS Microbiology Reviews·Irena LinhartováPeter Sebo
Apr 7, 2004·Biochemistry·Keisha G LangstonM Lou Ernst-Fonberg
Dec 26, 2001·Veterinary Microbiology·Sanjeev Kumar NarayananGeorge C Stewart
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·P StanleyC Hughes

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