Structure-function study of the amino-terminal stretch of the catalase subunit molecule in oligomerization, heme binding, and activity expression

Applied Microbiology and Biotechnology
M UedaA Tanaka

Abstract

Analysis of the protein structure of bovine liver catalase suggested that the N-terminal region containing two alpha-helices may function as a linker binding to another subunit. The number of amino-acid residues in catalase from the n-alkane-assimilating yeast Candida tropicalis (CTC) is the lowest of any eukaryotic catalase molecule hitherto investigated, and only one helix, corresponding to the helix alpha2 in bovine liver catalase, is estimated to be present in the same region. In the present study, N-terminal-deleted mutants of CTC were characterized to evaluate the role of the alpha-helix structure in the N-terminal region. CTCDelta1-4 and CTCDelta1-24, whose N-terminal regions were shortened by four and 24 amino-acid residues, respectively, showed an 80% decrease in specific activity compared to wild-type CTC in spite of containing the same amount of heme as in the wild-type. Polyacrylamide gel electrophoresis under nondenaturing conditions revealed that the mutants contained large amounts of oligomeric forms with molecular masses less than 220 kDa (tetramer assembly). Although the smaller oligomers were found to be bound with heme, only the tetramer exhibited catalase activity in activity staining on nondenaturing gel. C...Continue Reading

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Citations

Jul 1, 2005·Indian Journal of Clinical Biochemistry : IJCB·Prashen ChelikaniT M Radhakrishnan
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Randall J DeJongCarolina Barillas-Mury
Jan 6, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Tiffany VinckxPierre Cornelis
Mar 2, 2016·Free Radical Biology & Medicine·Olga RafikovaStephen M Black
Feb 11, 2015·Free Radical Biology & Medicine·Ritu ChakravartiDennis J Stuehr
Sep 12, 2018·Bioscience Reports·Véronique Larosa, Claire Remacle
May 14, 2011·Metallomics : Integrated Biometal Science·Pierre CornelisTiffany Vinckx

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