Structure of a highly acidic β-lactamase from the moderate halophile Chromohalobacter sp. 560 and the discovery of a Cs(+)-selective binding site

Acta Crystallographica. Section D, Biological Crystallography
Shigeki AraiRyota Kuroki

Abstract

Environmentally friendly absorbents are needed for Sr(2+) and Cs(+), as the removal of the radioactive Sr(2+) and Cs(+) that has leaked from the Fukushima Nuclear Power Plant is one of the most important problems in Japan. Halophilic proteins are known to have many acidic residues on their surface that can provide specific binding sites for metal ions such as Cs(+) or Sr(2+). The crystal structure of a halophilic β-lactamase from Chromohalobacter sp. 560 (HaBLA) was determined to resolutions of between 1.8 and 2.9 Å in space group P31 using X-ray crystallography. Moreover, the locations of bound Sr(2+) and Cs(+) ions were identified by anomalous X-ray diffraction. The location of one Cs(+)-specific binding site was identified in HaBLA even in the presence of a ninefold molar excess of Na(+) (90 mM Na(+)/10 mM Cs(+)). From an activity assay using isothermal titration calorimetry, the bound Sr(2+) and Cs(+) ions do not significantly affect the enzymatic function of HaBLA. The observation of a selective and high-affinity Cs(+)-binding site provides important information that is useful for the design of artificial Cs(+)-binding sites that may be useful in the bioremediation of radioactive isotopes.

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Citations

Jun 5, 2021·Biophysical Journal·Hosein Geraili Daronkola, Ana Vila Verde
Nov 6, 2021·Journal of Synchrotron Radiation·Akio YoneyamaYoshiki Seno

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Methods Mentioned

BETA
X-ray
deamidation
isothermal titration
PLAP
enzymatic assay

Software Mentioned

FFT
Origin
LSQKAB
HKL
HaBLA
2000
PDBeFold
SFALL
CCP
SIGMAA

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