Structure of a membrane-based steric chaperone in complex with its lipase substrate

Nature Structural & Molecular Biology
Kris PauwelsPatrick Van Gelder

Abstract

Secretion via the type II secretion pathway in Gram-negative bacteria often relies crucially on steric chaperones in the periplasm. Here, we report the crystal structure of the soluble form of a lipase-specific foldase (Lif) from Burkholderia glumae in complex with its cognate lipase. The structure reveals how Lif uses a novel alpha-helical scaffold to embrace lipase, thereby creating an unusually extensive folding platform.

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Citations

Jul 28, 2007·Extremophiles : Life Under Extreme Conditions·Hiroyasu OginoHaruo Ishikawa
Jul 31, 2013·Protein Expression and Purification·Ksenia Novototskaya-VlasovaMikhail Kirpichnikov
Apr 3, 2012·Nature Reviews. Microbiology·Konstantin V KorotkovWim G J Hol
Jan 1, 2008·Journal of Bacteriology·Joanna B GoldbergPierre Cornelis
Dec 17, 2014·Microbial Cell Factories·Viviane Paula MartiniNadia Krieger
Nov 17, 2012·International Journal of Biological Macromolecules·Xiaomei ZhengYunliu Fan
Apr 10, 2008·Protein Expression and Purification·Kris Pauwels, Patrick Van Gelder
Nov 28, 2006·Protein Expression and Purification·Hyo-Jung ParkHyung-Kwoun Kim
May 16, 2007·Molecular Microbiology·Kris PauwelsPatrick Van Gelder
Oct 6, 2006·Molecular Microbiology·Anastasias EconomouAnthony P Pugsley
Jan 7, 2014·Biochimica Et Biophysica Acta·Mangayarkarasi Nivaskumar, Olivera Francetic
May 15, 2013·Journal of Biotechnology·Hiroyasu OginoNoriyuki Doukyu
Mar 8, 2017·Infection and Immunity·Nicholas P Cianciotto, Richard C White
May 10, 2017·Molecular Microbiology·Jenny-Lee ThomassinOlivera Francetic
Mar 25, 2017·Frontiers in Bioengineering and Biotechnology·Faez Iqbal KhanYonghua Wang
Dec 7, 2020·International Journal of Biological Macromolecules·Yoshiaki YasutakeTomohiro Tamura
Feb 16, 2019·EcoSal Plus·Konstantin V Korotkov, Maria Sandkvist

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