PMID: 9557730Apr 29, 1998Paper

Structure of a neutralizing antibody bound monovalently to human rhinovirus 2

Journal of Virology
Elizabeth A HewatD Blaas

Abstract

The structure of a complex between human rhinovirus 2 (HRV2) and the Fab fragment of neutralizing monoclonal antibody (MAb) 3B10 has been determined to 25-A resolution by cryoelectron microscopy and three-dimensional reconstruction techniques. The footprint of 3B10 on HRV2 is very similar to that of neutralizing MAb 8F5, which binds bivalently across the icosahedral twofold axis. However, the 3B10 Fab fragment (Fab-3B10) is bound in an orientation, inclined at approximately 45 degrees to the surface of the virus capsid, which is compatible only with monovalent binding of the antibody. The canyon around the fivefold axis is not directly obstructed by the bound Fab. The X-ray structures of a closely related HRV (HRV1A) and a Fab fragment were fitted to the density maps of the HRV2-Fab-3B10 complex obtained by cryoelectron microscope techniques. The footprint of 3B10 on the viral surface is largely on VP2 but also covers the VP3 loop centered on residue 3064 and the VP1 loop centered on residue 1267. MAb 3B10 can interact directly with VP2 residue 2164, the site of an escape mutation on VP2, and with VP1 residues 1264 to 1267, the site of a deletion escape mutation. Deletion of these residues shortens the VP1 loop, moving it away ...Continue Reading

References

Apr 1, 1992·Journal of Virology·K C McCulloughU Kihm
Jun 1, 1990·The Journal of General Virology·G AppleyardJ Vadolas
Mar 10, 1989·Cell·J M GreveA McClelland
Nov 5, 1989·Journal of Molecular Biology·S S KimM A McKinlay
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R A Crowther
Sep 15, 1993·Structure·M A OliveiraM A McKinlay
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·F HoferD Blaas
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T J SmithT S Baker
Jan 1, 1996·Journal of Structural Biology·S D FullerT S Baker
Oct 15, 1996·Structure·R ZhaoM G Rossmann

❮ Previous
Next ❯

Citations

Mar 18, 2004·Reviews in Medical Virology·Nigel J Dimmock, Sam A Hardy
Mar 27, 2009·Journal of Virology·Susan HafensteinMichael G Rossmann
Nov 28, 2014·Journal of Virology·Kristin L ShinglerSusan Hafenstein
Mar 3, 2004·Journal of Virology·Günther BaravalleRenate Fuchs
Jun 21, 2011·Omics : a Journal of Integrative Biology·Fabio BagnoliRino Rappuoli
Dec 12, 2012·Advances in Virology·Renate Fuchs, Dieter Blaas
Jul 11, 2014·Nature Communications·Johannes PreinerPeter Hinterdorfer
Jun 24, 2020·Vaccines·Petra PazderovaKatarzyna Niespodziana
Aug 20, 2002·The Journal of General Virology·P J Klasse, Q J Sattentau
Jul 12, 2007·Journal of Separation Science·Leopold KremserErnst Kenndler
Jul 15, 2015·Journal of Virology·Shabih ShakeelSarah J Butcher
Mar 7, 2021·Viruses·Lila TouabiGary R McLean
Jul 25, 2000·Journal of Molecular Biology·N VerdaguerI Fita
May 1, 1999·Journal of Structural Biology·N Volkmann, D Hanein
May 18, 1999·Journal of Molecular Biology·M ValleJ L Carrascosa
Aug 19, 2016·Journal of Virology·Lindsey J OrgantiniSusan Hafenstein

❮ Previous
Next ❯

Related Concepts

Related Feeds