Structure of a Nudix protein from Pyrobaculum aerophilum reveals a dimer with two intersubunit beta-sheets

Acta Crystallographica. Section D, Biological Crystallography
Shuishu WangDavid Eisenberg

Abstract

Nudix proteins, formerly called MutT homolog proteins, are a large family of proteins that play an important role in reducing the accumulation of potentially toxic compounds inside the cell. They hydrolyze a wide variety of substrates that are mainly composed of a nucleoside diphosphate linked to some other moiety X and thus are called Nudix hydrolases. Here, the crystal structure of a Nudix hydrolase from the hyperthermophilic archaeon Pyrobaculum aerophilum is reported. The structure was determined by the single-wavelength anomalous scattering method with data collected at the peak anomalous wavelength of an iridium-derivatized crystal. It reveals an extensive dimer interface, with each subunit contributing two strands to the beta-sheet of the other subunit. Individual subunits consist of a mixed highly twisted and curved beta-sheet of 11 beta-strands and two alpha-helices, forming an alpha-beta-alpha sandwich. The conserved Nudix box signature motif, which contains the essential catalytic residues, is located at the first alpha-helix and the beta-strand and loop preceding it. The unusually short connections between secondary-structural elements, together with the dimer form of the structure, are likely to contribute to the t...Continue Reading

Citations

Apr 30, 2003·Journal of Structural Biology·Celia W Goulding, L Jeanne Perry
Jul 20, 2011·Nucleic Acids Research·Takao ArimoriYuriko Yamagata
Sep 10, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Garry W BuchkoPeter J Myler
Sep 25, 2010·PLoS Computational Biology·Cameron MuraMichael R Sawaya
Jun 21, 2005·Biochemical and Biophysical Research Communications·Qing-Chuan ZhengChia-Chung Sun
Aug 23, 2005·PLoS Biology·Morgan BeebyTodd O Yeates
Feb 10, 2009·Annals of Botany·Shin-nosuke HashidaHirofumi Uchimiya
Dec 10, 2016·Proteins·John R SroujiSteven E Brenner
May 5, 2004·Journal of Molecular Biology·Wasantha RanatungaStephen R Holbrook
Dec 8, 2004·Archives of Biochemistry and Biophysics·A S MildvanL M Amzel

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