Structure of a putative trans-editing enzyme for prolyl-tRNA synthetase from Aeropyrum pernix K1 at 1.7 A resolution

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Kazutaka MurayamaShigeyuki Yokoyama

Abstract

The crystal structure of APE2540, the putative trans-editing enzyme ProX from Aeropyrum pernix K1, was determined in a high-throughput manner. The crystal belongs to the monoclinic space group P2(1), with unit-cell parameters a = 47.4, b = 58.9, c = 53.6 A, beta = 106.8 degrees. The structure was solved by the multiwavelength anomalous dispersion method at 1.7 A and refined to an R factor of 16.8% (Rfree = 20.5%). The crystal structure includes two protein molecules in the asymmetric unit. Each monomer consists of eight beta-strands and seven alpha-helices. A structure-homology search revealed similarity between the trans-editing enzyme YbaK (or cysteinyl-tRNAPro deacylase) from Haemophilus influenzae (HI1434; 22% sequence identity) and putative ProX proteins from Caulobacter crescentus (16%) and Agrobacterium tumefaciens (21%).

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Mar 1, 1990·Journal of Molecular Graphics·G Vriend
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 21, 1998·Nucleic Acids Research·L Holm, C Sander
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Jun 26, 1999·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Y KawarabayasiH Kikuchi
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Satwik KamtekarThomas A Steitz
Jun 26, 2003·Nucleic Acids Research·Takeshi Kawabata
Dec 4, 2003·Journal of Synchrotron Radiation·Richard J MorrisVictor S Lamzin
Dec 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ivan AhelDieter Söll
Dec 19, 2003·Nucleic Acids Research·Alex BatemanSean R Eddy
Aug 24, 2004·The Journal of Biological Chemistry·Songon An, Karin Musier-Forsyth

❮ Previous
Next ❯

Citations

Jul 20, 2011·The Journal of Biological Chemistry·Byung Ran SoKarin Musier-Forsyth
Apr 9, 2013·The Journal of Biological Chemistry·Oscar Vargas-Rodriguez, Karin Musier-Forsyth
Nov 29, 2012·The Journal of Physical Chemistry. B·Sandeep KumarKarin Musier-Forsyth

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Ivan AhelDieter Söll
Proceedings of the National Academy of Sciences of the United States of America
Dragana KorencicDieter Söll
© 2021 Meta ULC. All rights reserved