Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore

Acta Crystallographica. Section D, Biological Crystallography
Nadezhda V PletnevaVladimir Z Pletnev

Abstract

The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRF...Continue Reading

Citations

Mar 28, 2008·Biochemistry·Alexander S MishinVladislav V Verkhusha
Feb 4, 2010·Journal of the American Chemical Society·Sergei PletnevVladislav V Verkhusha
Apr 28, 2009·Nature Chemical Biology·Alexey M BogdanovKonstantin A Lukyanov
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Roberto A ChicaStephen L Mayo
Jun 25, 2014·PloS One·Sergei PletnevVladislav V Verkhusha
Oct 29, 2018·Protein Science : a Publication of the Protein Society·So Eun KimKi Hyun Nam
Jun 5, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Elena A ProtasovaAlexey M Bogdanov
Mar 12, 2011·The Journal of Organic Chemistry·Pavel E IvashkinIlia V Yampolsky
Mar 1, 2007·Journal of the American Chemical Society·David P BarondeauElizabeth D Getzoff
Jan 27, 2007·Journal of the American Chemical Society·Seth Olsen, Sean C Smith

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