Structure of a sugar chain of a protease inhibitor isolated from barbados pride (Caesalpinia pulcherrima Sw.) seeds

Journal of Biochemistry
S HaseT Ikenaka

Abstract

An asparagine-linked sugar chain of a protease inhibitor from barbados pride (Caesalpinia pulcherrima Sw.) was liberated by hydrazinolysis. After N-acetylation, the reducing end residue of this carbohydrate unit was coupled with 2-aminopyridine and the pyridylamino (PA-) derivative was purified by gel-filtration and reversed-phase HPLC. The structure of the resulting PA-sugar chain was determined mainly by stepwise exoglycosidase digestions and 500 MHz 1H-NMR spectroscopy and proved to be as follows: (formula; see text).

Citations

Jul 31, 1990·European Journal of Biochemistry·M HayashiT Akazawa
May 31, 1990·European Journal of Biochemistry·J B BouwstraJ F Vliegenthart
Oct 25, 1996·The Journal of Biological Chemistry·H OhsugaY C Lee
Aug 14, 1999·European Journal of Biochemistry·Y KimuraA Sturm
May 1, 1993·European Journal of Biochemistry·V KubelkaJ F Vliegenthart
Jul 6, 2005·Chemical Communications : Chem Comm·Jun Zhe MinTakeshi Fukushima
Aug 28, 1990·European Journal of Biochemistry·D Balshüsemann, L Jaenicke
Mar 3, 1999·Bioscience, Biotechnology, and Biochemistry·N Q PalacpacT Seki
Nov 27, 2016·Analytical and Bioanalytical Chemistry·Gerda C M Vreeker, Manfred Wuhrer
Aug 1, 1992·European Journal of Biochemistry·E StaudacherL März
Dec 16, 2020·Phytochemistry·Ilana Cruz-SilvaMariana da Silva Araujo
Nov 1, 1994·Phytochemistry·L Wan, R B van Huystee
Feb 14, 2002·Chemical Reviews·Yehia Mechref, Milos V Novotny
Nov 15, 1990·Analytical Biochemistry·T NakamuraM Endo

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