Abstract
Surfactant protein D (SP-D) is a lung-specific protein that is synthesized and secreted by lung epithelial cells and is believed to play an important role in lung host defence. This protein belongs to the C-type lectin family, which is characterized by an N-terminal cysteine-rich domain, a collagen-like domain, a neck domain and a carbohydrate recognition domain (CRD). To elucidate the biological actions of this animal lectin against such pathogens as micro-organisms, the biological activities of a recombinant partial SP-D lacking a collagen-like domain were examined. A recombinant human SP-D, consisting of a short collagen region (two repeats of Gly-Xaa-Yaa amino acid sequences), the neck domain and the CRD, was expressed in Escherichia coli. The recombinant SP-D was purified on a nickel column and then on a maltose-agarose column. This protein can form a trimeric structure owing to the neck domain and exhibits sugar-binding activity and specificity similar to those of native human SP-D. The recombinant SP-D caused dose-dependent and calcium-dependent agglutination of E. coli Y1088. The agglutination titre (the concentration required to achieve a 50% decrease in light transmission by agglutination) of recombinant SP-D was appr...Continue Reading
Citations
Nov 13, 2008·The Journal of Allergy and Clinical Immunology·Angela Haczku
May 5, 2007·Molecular Immunology·Astrid HogenkampHenk P Haagsman
Dec 21, 2006·Microbiology and Immunology·Hiroyuki KeshiNobutaka Wakamiya
Oct 18, 2005·Pharmacology & Therapeutics·Angela Haczku
Jun 3, 2008·Biochimica Et Biophysica Acta·Jesús Pérez-Gil
Jun 6, 2012·Journal of Biomedicine & Biotechnology·Wy Ching NgPatrick C Reading
Feb 8, 2018·The Journal of Biological Chemistry·Sarah Sze Wah WongVishukumar Aimanianda
Aug 14, 1999·Immunology·T KaseN Wakamiya
Mar 7, 2007·The Journal of Veterinary Medical Science·Takao KawaiNobutaka Wakamiya
Aug 28, 1998·Bioscience, Biotechnology, and Biochemistry·S EdaN Wakamiya
Nov 29, 2008·Applied Microbiology and Biotechnology·Natsuko MiuraMitsuyoshi Ueda
Mar 7, 2006·Free Radical Research·Vitaliy StarostaMatthias Griese
May 1, 1999·The Journal of Biological Chemistry·K OhtaniN Wakamiya
Sep 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emma R JobPatrick C Reading
Feb 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emma R JobPatrick C Reading
Jan 14, 1999·The American Journal of Physiology·G Z CaiM A Moxley
Aug 5, 1998·The American Journal of Physiology·R J MasonD R Voelker
Sep 7, 2011·Journal of Biochemistry·Takayuki YoshizakiNobutaka Wakamiya
Jan 27, 2004·Infection and Immunity·Rania JounblatPeter W Andrew
Jan 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·K L HartshornE C Crouch
Mar 28, 2002·The Journal of Biological Chemistry·Hirofumi ChibaDennis R Voelker
Feb 18, 1999·Journal of Immunological Methods·K OhtaniN Wakamiya