PMID: 9428716Jan 15, 1998Paper

Structure of an acidic exopolysaccharide of Burkholderia pseudomallei

European Journal of Biochemistry
M NimtzI Steinmetz

Abstract

A recently described water-soluble exopolysaccharide of Burkholderia pseudomallei recognized by the IgG 1 monoclonal antibody 3015 [Steinmetz, I., Rohde, M. & Brenneke, B. (1995) Infect. Immun. 63, 3959-3965] was isolated by repetitive ethanol-precipitation steps and by anion-exchange chromatography. The structure of the polysaccharide was determined by a combination of chemical-derivatization and mass-spectrometric techniques (compositional and methylation analysis, GC/MS, and electrospray-ionization-MS/MS of reduced and permethylated hydrolytic fragments), and two-dimensional 1H-NMR methods (COSY, TOCSY and NOESY) and confirmed by isolation and structural characterization of the depolymerized repeating unit of the polysaccharide. The combined structural data established a linear tetrasaccharide repeating unit consisting of three galactose residues, one bearing a 2-linked O-acetyl group, and a 3-deoxy-D-manno-2-octulosonic acid residue. [-->3)-beta-D-Galp2Ac-(1-->4)-alpha-D-Galp-(1-->3)-beta-D-Galp-(1- ->5)-beta-Kdo-(2-->]n

References

Sep 2, 1992·Carbohydrate Research·Y A KnirelV I Efremenko
Feb 15, 1992·European Journal of Biochemistry·E AltmanM B Perry
Jan 1, 1990·Current Topics in Microbiology and Immunology·H J Jennings
Jan 1, 1990·Current Topics in Microbiology and Immunology·B Jann, K Jann
Jan 1, 1990·Current Topics in Microbiology and Immunology·E R Moxon, J S Kroll
May 1, 1989·The Journal of Infectious Diseases·W ChaowagulN Pitakwatchara

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Citations

Feb 16, 2000·Acta Tropica·P J Brett, D E Woods
Feb 16, 2000·Acta Tropica·I SteinmetzB Brenneke
Oct 26, 2000·Trends in Genetics : TIG·J RoyoG Hueros
May 15, 2002·Microbiology and Immunology·Narisara Anuntagool, Stitaya Sirisinha
Apr 3, 2001·Journal of Clinical Microbiology·T Schülin, I Steinmetz
Nov 22, 2013·PloS One·Mercedes Gonzalez-JuarreroTorsten M Eckstein
Feb 5, 2011·The American Journal of Tropical Medicine and Hygiene·Dionne Bezerra RolimAparecida Tiemi Nagao-Dias
Aug 24, 2010·Clinical Therapeutics·Mitali Sarkar-Tyson, Richard W Titball
Aug 24, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·H NeubauerH C Scholz
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Luigi ChiariniRoberto Rizzo
Sep 15, 2016·The Journal of Organic Chemistry·Marcelina MazurCharles Gauthier
Aug 21, 2010·Journal of Medical Microbiology·Shauna L Reckseidler-ZentenoDonald E Woods
Jul 24, 2007·Journal of Medical Microbiology·M Sarkar-TysonR W Titball
Feb 11, 2021·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Kevin Muru, Charles Gauthier
Jul 15, 2020·Organic Letters·Oscar Javier Gamboa MarinCharles Gauthier

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