Structure of Bordetella pertussis virulence factor P.69 pertactin

Nature
P EmsleyN W Isaacs

Abstract

A new generation of whooping-cough vaccines contain P.69 pertactin, a surface-exposed domain of an outer membrane protein expressed by the virulent bacterium Bordetella pertussis. This protein is a virulence factor that mediates adhesion to target mammalian cells, a reaction that is in part mediated by an RGD sequence. The X-ray crystal structure of P.69 pertactin has been determined to 2.5 A. The protein fold consists of a 16-stranded parallel beta-helix with a V-shaped cross-section, and is the largest beta-helix known to date. Several between-strand weakly conserved amino-acid repeats form internal and external ladders. The structure appears as a helix from which several loops protrude, which contain sequence motifs associated with the biological activity of the protein. One particular (GGXXP)5 sequence is located directly after the RGD motif, and may mediate interaction with epithelial cells. The carboxy-terminal region of P.69 pertactin incorporates a (PQP)5 motif loop containing the major immunoprotective epitope.

References

Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E LeiningerM J Brennan
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I G CharlesN F Fairweather
Jan 1, 1985·Methods in Enzymology·B C Wang
Jan 15, 1994·The Biochemical Journal·M P Williamson
Feb 8, 1996·The New England Journal of Medicine·L GustafssonJ Storsaeter
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

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Citations

Jul 9, 1998·Protein Science : a Publication of the Protein Society·A BatemanS A Teichmann
Mar 11, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kaoru NishimuraJeremy R H Tame
Apr 7, 2005·Journal of Molecular Biology·Andrey V KajavaAlasdair C Steven
Jan 24, 2004·Microbes and Infection·Hartmut H NiemannDirk W Heinz
Apr 10, 2013·Research in Microbiology·Jan GrijpstraJan Tommassen
Apr 23, 2004·Current Opinion in Structural Biology·Robert Tycko
Apr 20, 2004·Current Opinion in Structural Biology·Han Remaut, Gabriel Waksman
Dec 19, 2001·Progress in Biophysics and Molecular Biology·J Jenkins, R Pickersgill
Oct 28, 1998·Molecular Aspects of Medicine·G Del GiudiceR Rappuoli
May 12, 2001·FEMS Microbiology Reviews·A M SmithM J Walker
Dec 20, 1996·Molecular and Cellular Endocrinology·J J RemyJ M Bidart
Jun 1, 1997·Current Opinion in Structural Biology·J Heringa, W R Taylor
Feb 24, 2001·Trends in Microbiology·F Jacob-DubuissonC Locht
Feb 15, 2001·Current Opinion in Microbiology·C LochtF Jacob-Dubuisson
Dec 1, 1996·Nature Structural Biology·B Kobe
Nov 13, 2007·Nature Structural & Molecular Biology·Travis J BarnardSusan K Buchanan
Sep 20, 2005·Nature Structural & Molecular Biology·Stephen A McMahonPartho Ghosh
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Janine H PetersonHarris D Bernstein
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Olga PavlovaHarris D Bernstein
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·S SteinbacherR Huber
Apr 29, 2009·The Journal of Biological Chemistry·Marie-Ève CharbonneauMichael Mourez
Oct 19, 2011·The Journal of Biological Chemistry·Denisse L LeytonIan R Henderson
May 23, 2002·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Lenore CowenBonnie Berger
Nov 4, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C M ThomasJ D Jones
Jul 11, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yanshi ZhuNeil W Isaacs
May 30, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Li ZhaoMichael E P Murphy
Feb 24, 2001·Protein Science : a Publication of the Protein Society·J F KreisbergJ King
Jun 24, 2011·Clinical and Vaccine Immunology : CVI·Eleanor WatsonDavid G E Smith
Apr 28, 2010·Infection and Immunity·Carol S InatsukaPeggy A Cotter
Feb 17, 2001·Infection and Immunity·I R Henderson, J P Nataro
Sep 24, 2004·Infection and Immunity·Maria Kostakioti, Christos Stathopoulos

❮ Previous
Next ❯

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