PMID: 37233Jun 1, 1979

Structure of common pili from Escherichia coli

Journal of Bacteriology
J C McMichael, J T Ou

Abstract

Several important properties of the common pili from Escherichia coli are discussed. These pili were resistant to the gentle Folin-Ciocalteau reagent methods for protein detection and were not readily solubilized by sodium dodecyl sulfate. They were found to contain a reducing sugar but not peptidoglycan. The pilin had multiple conformations in sodium dodecyl sulfate solution, and the appearance of multiple bands on sodium dodecyl sulfate gels did not necessarily indicate heterogeneity of the preparation. The ilus subunit was found to be a different protein than outer membrane III, which has the same apparent molecular weight. In addition, we conformed the results of Brinton (Trans. N.Y. Acad. Sci 27:1003-1054, 1965): that there is a dramatic change in the properties of pili after they are heated at pH values below 2.

Citations

Jun 1, 1991·Journal of Industrial Microbiology·K L Ogden, A L Taylor
Jan 1, 1990·Microbiology and Immunology·Y Honma, N Nakasone
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·E T SaulinoS J Hultgren
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·María A RendónJorge A Girón
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Christopher J AlteriRichard L Friedman
Jan 1, 1983·Critical Reviews in Microbiology·G W Jones, R E Isaacson
Jul 3, 1989·FEBS Letters·J Parkkinen, T K Korhonen
Jan 1, 1990·Microbiology and Immunology·J Aslanzadeh, L J Paulissen
Mar 30, 2010·Journal of Bacteriology·Karina Mazariego-EspinosaJuan Xicohtencatl-Cortes

Related Concepts

Bacterial Proteins
Sugars
Cell Wall
Alkalescens-Dispar Group
Hydrogen-Ion Concentration
Polypeptides

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