Structure of D-63 from sulfolobus spindle-shaped virus 1: surface properties of the dimeric four-helix bundle suggest an adaptor protein function

Journal of Virology
Paul KraftC Martin Lawrence

Abstract

Sulfolobus spindle-shaped virus 1 (SSV1) and its fusellovirus homologues can be found in many acidic (pH <or= 4.0) hot springs (>or=70 degrees C) around the world. SSV1 contains a 15.5-kb double-stranded DNA genome that encodes 34 proteins with greater than 50 amino acids. A site-specific integrase and a DnaA-like protein have been previously identified by sequence homology, and three structural proteins have been isolated from purified virus and identified by N-terminal sequencing (VP1, VP2, and VP3). The functions of the remaining 29 proteins are currently unknown. To assign functions to these proteins, we have initiated biochemical and structural studies on the SSV1 proteome. Here we report the structure of SSV1 D-63. The structure reveals a helix-turn-helix motif that dimerizes to form an antiparallel four-helix bundle. Mapping residues conserved among three fusellovirus isolates onto the structure shows that one face of the rod-shaped molecule is highly conserved. This conserved surface spans the dimer axis and thus exhibits 2-fold symmetry. Two smaller conserved patches, also related by 2-fold symmetry, are found on the opposite face of the molecule. All of these conserved surfaces are devoid of clefts or pockets typicall...Continue Reading

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Citations

Oct 3, 2013·Extremophiles : Life Under Extreme Conditions·Patrizia ContursiGabriella Fiorentino
Jun 7, 2006·Nature Reviews. Microbiology·Alice C OrtmannMark Young
Jan 23, 2009·The Journal of Biological Chemistry·C Martin LawrenceMark J Young
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Dec 7, 2014·Extremophiles : Life Under Extreme Conditions·Salvatore FuscoPatrizia Contursi
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Nov 26, 2010·Journal of Molecular Biology·Nathanael G LintnerC Martin Lawrence
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Dec 19, 2006·Virology·Adam J Clore, Kenneth M Stedman
Mar 6, 2007·Virology·Eric T LarsonC Martin Lawrence
Jan 7, 2006·Extremophiles : Life Under Extreme Conditions·Georg Lipps
Oct 8, 2019·Structure·Ross HartmanC Martin Lawrence

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