Structure of eIF4E in Complex with an eIF4G Peptide Supports a Universal Bipartite Binding Mode for Protein Translation

Plant Physiology
Manuel MirasJordi Querol-Audí

Abstract

The association-dissociation of the cap-binding protein eukaryotic translation initiation factor 4E (eIF4E) with eIF4G is a key control step in eukaryotic translation. The paradigm on the eIF4E-eIF4G interaction states that eIF4G binds to the dorsal surface of eIF4E through a single canonical alpha-helical motif, while metazoan eIF4E-binding proteins (m4E-BPs) advantageously compete against eIF4G via bimodal interactions involving this canonical motif and a second noncanonical motif of the eIF4E surface. Metazoan eIF4Gs share this extended binding interface with m4E-BPs, with significant implications on the understanding of translation regulation and the design of therapeutic molecules. Here we show the high-resolution structure of melon (Cucumis melo) eIF4E in complex with a melon eIF4G peptide and propose the first eIF4E-eIF4G structural model for plants. Our structural data together with functional analyses demonstrate that plant eIF4G binds to eIF4E through both the canonical and noncanonical motifs, similarly to metazoan eIF4E-eIF4G complexes. As in the case of metazoan eIF4E-eIF4G, this may have very important practical implications, as plant eIF4E-eIF4G is also involved in a significant number of plant diseases. In light...Continue Reading

Citations

Dec 15, 2017·Frontiers in Plant Science·Verónica TrunigerMiguel A Aranda
Jul 7, 2018·Annual Review of Phytopathology·Cristina RosaBryce W Falk
Jan 9, 2019·Phytopathology·Manuel MirasMiguel A Aranda
May 24, 2019·The FEBS Journal·Aaron N BrunsDavid M Bisaro
May 2, 2018·Scientific Reports·Nick ArndtMichael Altmann
Jun 22, 2019·Annual Review of Biochemistry·Jerry Pelletier, Nahum Sonenberg
Nov 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Luciana Coutinho de OliveiraKatherine L B Borden
Nov 6, 2020·Nucleic Acids Research·Ignacio Moya-RamírezKaren Polizzi
Nov 26, 2019·Biochemistry·Rishi Kumar MishraTanweer Hussain
Sep 21, 2021·Cellular and Molecular Life Sciences : CMLS·Alice RomagnoliDaniele Di Marino
Nov 11, 2021·The FEBS Journal·Mary Christie, Cátia Igreja

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