PMID: 69727Aug 1, 1977Paper

Structure of hepatitis B Dane particle DNA and nature of the endogenous DNA polymerase reaction

Journal of Virology
T A LandersW S Robinson

Abstract

The circular DNA of hepatitis B Dane particles, which serves as the primer/template for an endogenous DNA polymerase, was analyzed by electrophoresis before and after a polymerase reaction and after digestion by restriction endonuclease or single-strand-specific endonuclease S1. The unreacted molecules extracted from the particles were electrophoretically heterogeneous, and treatment with S1 nuclease produced double-stranded linear DNA ranging in length from 1,700 to 2,800 base pairs (bp). After an endogenous DNA polymerase reaction, two discrete species of DNA molecules were found: a circular form and a linear form 3,200 bp long. The reaction resulted in a population of molecules with an elongated and more homogeneous double-stranded region. These results suggest that the circular molecules in Dane particles have single-stranded regions of varying lengths that are made double stranded during the DNA polymerase reaction. The endogenous DNA polymerase was found to initiate apparently at random in a region spanning more than a third of the molecule. Analysis of restriction endonuclease cleavage fragments of the fully elongated DNA revealed that although the molecules were of a uniform length, they were somewhat heterogeneous in s...Continue Reading

References

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Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J SummersI Millman
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Oct 1, 1952·Proceedings of the Society for Experimental Biology and Medicine·R W MCCOLLUM

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Citations

Sep 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·O YokosukaJ Summers
Jun 1, 1980·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·E NordenfeltE Miller
Jan 1, 1980·Annals of the New York Academy of Sciences·W S Robinson
Jan 1, 1982·Journal of Medical Virology·M BerningerJ L Gerin
Mar 1, 1981·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J Summers
Mar 1, 1996·Journal of Viral Hepatitis·M J McGarveyH C Thomas
Jan 1, 1990·Journal of Clinical Laboratory Analysis·S KanekoR H Miller
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C Seeger, W S Mason
Jan 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W S RobinsonP L Marion
Jan 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P J KniskernR W Ellis
Dec 14, 2001·European Journal of Gastroenterology & Hepatology·L M WoltersR A de Man
Oct 1, 1983·Molecular and Cellular Biology·D N StandringW J Rutter
Jan 1, 1979·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·S Kit
Sep 1, 1991·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Oct 1, 1988·Journal of Virology·R SprengelH Will
Aug 1, 1983·Journal of Virology·H DeliusK Murray
Feb 1, 1980·Journal of Virology·P L MarionW S Robinson

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