Structure of (KIAGKIA)3 aggregates in phospholipid bilayers by solid-state NMR

Biophysical Journal
Orsolya TokeJacob Schaefer

Abstract

The interchain (13)C-(19)F dipolar coupling measured in a rotational-echo double-resonance (REDOR) experiment performed on mixtures of differently labeled KIAGKIA-KIAGKIA-KIAGKIA (K3) peptides (one specifically (13)C labeled, and the other specifically (19)F labeled) in multilamellar vesicles of dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol (1:1) shows that K3 forms close-packed clusters, primarily dimers, in bilayers at a lipid/peptide molar ratio (L/P) of 20. Dipolar coupling to additional peptides is weaker than that within the dimers, consistent with aggregates of monomers and dimers. Analysis of the sideband dephasing rates indicates a preferred orientation between the peptide chains of the dimers. The combination of the distance and orientation information from REDOR is consistent with a parallel (N-N) dimer structure in which two K3 helices intersect at a cross-angle of approximately 20 degrees. Static (19)F NMR experiments performed on K3 in oriented lipid bilayers show that between L/P = 200 and L/P = 20, K3 chains change their absolute orientation with respect to the membrane normal. This result suggests that the K3 dimers detected by REDOR at L/P = 20 are not on the surface of the bilayer but are...Continue Reading

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Citations

Feb 12, 2011·European Biophysics Journal : EBJ·Ralf HeinzmannAnne S Ulrich
Nov 15, 2011·European Biophysics Journal : EBJ·Parvesh WadhwaniAnne S Ulrich
Dec 25, 2007·Journal of Magnetic Resonance·Ulrich H N DürrAnne S Ulrich
Nov 18, 2008·Biochimica Et Biophysica Acta·David I FernandezFrances Separovic
Jun 17, 2006·Biochimica Et Biophysica Acta·Kaoru Nomura, Gerardo Corzo
May 24, 2006·Biochimica Et Biophysica Acta·Ulrich H N DürrAyyalusamy Ramamoorthy
Sep 30, 2006·Journal of Magnetic Resonance·Shigeru Matsuoka, Jacob Schaefer
Nov 13, 2013·Biochimica Et Biophysica Acta·Parvesh WadhwaniAnne S Ulrich
Dec 17, 2014·Biochimica Et Biophysica Acta·Julia MisiewiczAnne S Ulrich
Nov 5, 2016·Biochimica Et Biophysica Acta·Mariah J KingHee-Seung Lee
Jun 9, 2020·Frontiers in Cell and Developmental Biology·Juanjuan SuManuel N Melo
Oct 26, 2021·Chemical Reviews·Alexander A ShcherbakovMei Hong

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