Structure of Lung-Mimetic Multilamellar Bodies with Lipid Compositions Relevant in Pneumonia

Langmuir : the ACS Journal of Surfaces and Colloids
Dylan SteerCecilia Leal

Abstract

The hierarchical assembly of lipids, as modulated by composition and environment, plays a significant role in the function of biological membranes and a myriad of diseases. Elevated concentrations of calcium ions and cardiolipin (CL), an anionic tetra-alkyl lipid found in mitochondria and some bacterial cell membranes, have been implicated in pneumonia recently. However, their impact on the physicochemical properties of lipid assemblies in lungs and how it impairs alveoli function is still unknown. We use small- and wide-angle X-ray scattering (S/WAXS) and solid-state nuclear magnetic resonance (ssNMR) to probe the structure and dynamics of lung-mimetic multilamellar bodies (MLBs) in the presence of Ca2+ and CL. We conjecture that CL overexpressed in the hypophase of alveoli strongly affects the structure of lung-lipid bilayers and their stacking in the MLBs. Specifically, S/WAXS data revealed that CL induces significant shrinkage of the water-layer separating the concentric bilayers in multilamellar aggregates. ssNMR measurements indicate that this interbilayer tightening is due to undulation repulsion damping as CL renders the glycerol backbone of the membranes significantly more static. In addition to MLB dehydration, CL pro...Continue Reading

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Citations

Jan 17, 2020·Liquid Crystals Reviews·Marilyn Porras-Gomez, Cecilia Leal
Jan 17, 2020·Materials Horizons·Sarith R BandaraCecilia Leal
Feb 29, 2020·Nature Communications·Mijung KimCecilia Leal
Nov 18, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Joseph PazziAnand Bala Subramaniam
Jul 6, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Vaishnavi GirishAnand Bala Subramaniam
Nov 10, 2021·Chemical Reviews·Yoo Kyung Go, Cecilia Leal

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