Structure of mammalian eIF3 in the context of the 43S preinitiation complex

Nature
Amedee des GeorgesYaser Hashem

Abstract

During eukaryotic translation initiation, 43S complexes, comprising a 40S ribosomal subunit, initiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of messenger RNA and scan along it to the initiation codon. Scanning on structured mRNAs also requires the DExH-box protein DHX29. Mammalian eIF3 contains 13 subunits and participates in nearly all steps of translation initiation. Eight subunits having PCI (proteasome, COP9 signalosome, eIF3) or MPN (Mpr1, Pad1, amino-terminal) domains constitute the structural core of eIF3, to which five peripheral subunits are flexibly linked. Here we present a cryo-electron microscopy structure of eIF3 in the context of the DHX29-bound 43S complex, showing the PCI/MPN core at ∼6 Å resolution. It reveals the organization of the individual subunits and their interactions with components of the 43S complex. We were able to build near-complete polyalanine-level models of the eIF3 PCI/MPN core and of two peripheral subunits. The implications for understanding mRNA ribosomal attachment and scanning are discussed.

References

May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Jun 8, 2004·The Journal of Biological Chemistry·Zhiyi WeiWeimin Gong
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
May 14, 2005·Journal of Structural Biology·Christian SulowayBridget Carragher
Oct 1, 2005·Quarterly Reviews of Biophysics·Assen Marintchev, Gerhard Wagner
Jun 13, 2006·The Journal of Biological Chemistry·Aaron K LeFebvreRobert E Rhoads
Aug 22, 2006·Trends in Biochemical Sciences·Alan G Hinnebusch
Jun 22, 2007·The EMBO Journal·Mamiko MasutaniHiroaki Imataka
Dec 15, 2007·Nature Protocols·Robert A GrassucciJoachim Frank
May 10, 2008·The EMBO Journal·Andrey V PisarevTatyana V Pestova
Jul 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Min ZhouCarol V Robinson
Dec 17, 2009·Methods in Molecular Biology·Joshua E Elias, Steven P Gygi
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Richard J JacksonTatyana V Pestova
Feb 11, 2011·Nucleic Acids Research·Yingpu YuChristopher U T Hellen
Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chaomin SunJamie H D Cate
Jan 13, 2012·Nature·Gabriel C LanderAndreas Martin
Mar 27, 2012·Nature Structural & Molecular Biology·Emmanuelle SchmittYves Mechulam
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Florian BeckFriedrich Förster
Sep 11, 2012·Bioarchitecture·Andrew M Ellisdon, Murray Stewart
Sep 25, 2012·Journal of Structural Biology·Sjors H W Scheres
Oct 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Vidya DhoteTatyana V Pestova
Apr 30, 2013·Structure·Jordi Querol-AudiEva Nogales
May 3, 2013·Nature·Andreas M AngerRoland Beckmann
Aug 8, 2013·The Journal of Biological Chemistry·Zizheng DongJian-Ting Zhang

❮ Previous
Next ❯

Citations

Jan 20, 2016·Nucleic Acids Research·Irina S AbaevaChristopher U T Hellen
Nov 26, 2015·Cell·Kate D MeyerSamie R Jaffrey
May 9, 2016·Current Opinion in Cell Biology·Rebecca M Voorhees, Ramanujan S Hegde
May 24, 2016·Structure·M Duane SmithJamie H Cate
Aug 18, 2016·Nucleic Acids Research·Natacha LarburuCélia Plisson-Chastang
Aug 10, 2016·Bioinformatics·Gianluca CorradoAndrea Passerini
Nov 9, 2016·Nature Communications·Kristin Kiosze-BeckerRobert Tampé
Feb 1, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christopher H S Aylett, Nenad Ban
Feb 1, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alex G JohnsonJoseph D Puglisi
Feb 1, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jamie H D Cate
Jul 27, 2017·RNA Biology·Vladislava HronováLeoš Shivaya Valášek
Feb 6, 2018·Nucleic Acids Research·Boris EliseevChristiane Schaffitzel
Nov 15, 2017·Nucleic Acids Research·Alex G JohnsonJoseph D Puglisi
Oct 6, 2017·Nucleic Acids Research·Leoš Shivaya ValášekStanislava Gunišová
Apr 7, 2018·Wiley Interdisciplinary Reviews. RNA·Nikolay E Shirokikh, Thomas Preiss
Feb 24, 2018·Biochemical Society Transactions·Miriam WaldenElton Zeqiraj
Jul 19, 2018·Yeast·Robert A Crawford, Graham D Pavitt
May 8, 2018·Cold Spring Harbor Perspectives in Biology·Christopher U T Hellen
May 8, 2018·Cold Spring Harbor Perspectives in Biology·William C Merrick, Graham D Pavitt
Jun 16, 2018·Cold Spring Harbor Perspectives in Biology·Amy JobeJoachim Frank
Nov 4, 2017·RNA Biology·Andreia Gomes-DuarteLuísa Romão
Aug 23, 2019·International Journal of Molecular Sciences·Yoshika JanapalaNikolay E Shirokikh
Oct 24, 2019·Nucleic Acids Research·Kristýna PoncováLeoš Shivaya Valášek
Feb 17, 2017·Biochemical Society Transactions·Carol V Robinson
Feb 24, 2019·International Journal of Molecular Sciences·Emmanuelle SchmittYves Mechulam
Sep 19, 2019·The Journal of Cell Biology·Mary Kate BonnerAlexander E Kelly
Jan 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Bridget Y Huang, Israel S Fernández
Mar 6, 2018·Science Advances·Matthew M ParksScott C Blanchard
Feb 8, 2020·Cellular and Molecular Life Sciences : CMLS·Sihyeon AnSung Key Jang
Dec 4, 2016·Cellular and Molecular Life Sciences : CMLS·Rafaela LacerdaLuísa Romão

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.