Structure of Mesorhizobium loti arylamine N-acetyltransferase 1

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Simon HoltonE Sim

Abstract

The arylamine N-acetyltransferase (NAT) enzymes have been found in a broad range of both eukaryotic and prokaryotic organisms. The NAT enzymes catalyse the transfer of an acetyl group from acetyl Co-enzyme A onto the terminal nitrogen of a range of arylamine, hydrazine and arylhydrazine compounds. Recently, several NAT structures have been reported from different prokaryotic sources including Salmonella typhimurium, Mycobacterium smegmatis and Pseudomonas aeruginosa. Bioinformatics analysis of the Mesorhizobium loti genome revealed two NAT paralogues, the first example of multiple NAT isoenzymes in a eubacterial organism. The M. loti NAT 1 enzyme was recombinantly expressed and purified for X-ray crystallographic studies. The purified enzyme was crystallized in 0.5 M Ca(OAc)2, 16% PEG 3350, 0.1 M Tris-HCl pH 8.5 using the sitting-drop vapour-diffusion method. A data set diffracting to 2.0 A was collected from a single crystal at 100 K. The crystal belongs to the orthorhombic spacegroup P2(1)2(1)2(1), with unit-cell parameters a = 53.2, b = 97.3, c = 114.3 A. The structure was refined to a final free-R factor of 24.8%. The structure reveals that despite low sequence homology, M. loti NAT1 shares the common fold as reported in pr...Continue Reading

References

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Jan 1, 1989·Pharmacology & Therapeutics·D A Evans
May 20, 1999·Nature Structural Biology·A PerrakisV S Lamzin
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Apr 23, 2002·Pharmacogenomics·Frédérique PompeoEdith Sim

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Citations

Oct 3, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Benjamin PluvinageFernando Rodrigues-Lima
Mar 21, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jason M WalravenDavid W Hein
Jun 19, 2012·Journal of Bacteriology·Debashree ChatterjeeHolger Sondermann
Mar 22, 2007·BMC Biochemistry·Isaac M Westwood, Edith Sim
Apr 13, 2007·Expert Opinion on Drug Metabolism & Toxicology·Edith SimElizabeth Fullam
Mar 23, 2013·British Journal of Pharmacology·Xiaotong ZhouBaojian Wu
Apr 1, 2008·Biochemical and Biophysical Research Communications·Robert P EricksonYoichi Gondo
Dec 23, 2008·Chembiochem : a European Journal of Chemical Biology·Xiumei WuTaifo Mahmud
Nov 2, 2019·World Journal of Microbiology & Biotechnology·Vasiliki GarefalakiSotiria Boukouvala
Jul 22, 2021·Applied and Environmental Microbiology·Vasiliki GarefalakiSotiria Boukouvala

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