Structure of MFP2 and its function in enhancing MSP polymerization in Ascaris sperm amoeboid motility

Journal of Molecular Biology
Richard P GrantMurray Stewart

Abstract

The simplicity and specialization of the cell motility machinery of Ascaris sperm provides a powerful system in which to probe the basic molecular mechanism of amoeboid cell motility. Although Ascaris sperm locomotion closely resembles that seen in many other types of crawling cell, movement is generated by modulation of a cytoskeleton based on the major sperm protein (MSP) rather than the actin present in other cell types. The Ascaris motility machinery can be studied conveniently in a cell-free in vitro system based on the movement of plasma membrane vesicles by fibres constructed from bundles of MSP filaments. In addition to ATP, MSP and a plasma membrane protein, reconstitution of MSP motility in this cell-free extract requires cytosolic proteins to orchestrate the site-specific assembly and bundling of MSP filaments that generates locomotion. One of these proteins, MFP2, accelerates the rate of movement in this assay. Here, we describe crystal structures of two isoforms of MFP2 and show that both are constructed from two domains that have the same fold based on a novel, compact beta sheet arrangement. Patterns of conservation observed in a structure-based analysis of MFP2 sequences from different nematode species identifie...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Mar 1, 1970·Journal of Molecular Biology·S B Needleman, C D Wunsch
Jan 12, 1996·Cell·J A Theriot
Jul 12, 1996·Journal of Molecular Biology·A HaafM Stewart
Oct 25, 1996·Journal of Molecular Biology·T L BullockM Stewart
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Apr 1, 1997·Journal of Molecular Graphics & Modelling·R M Esnouf
Mar 21, 1998·Nature Structural Biology·T L BullockM Stewart
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D MullinsT D Pollard
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Nov 13, 1999·Nature Cell Biology·C J MerrifieldW Almers
Feb 19, 2000·Current Opinion in Cell Biology·G G Borisy, T M Svitkina
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·T D PollardR D Mullins
Mar 7, 2001·Current Biology : CB·L A CameronG G Borisy
May 31, 2001·Science·D PantaloniM F Carlier
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Mar 26, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marie-France CarlierDominique Pantaloni
Sep 19, 2003·Current Biology : CB·Alex Mogilner, George Oster
Oct 17, 2003·Current Biology : CB·Sally H ZigmondMartin Pring
Nov 25, 2003·Science·Alex Mogilner, George Oster
Nov 25, 2003·Science·Long MiaoThomas M Roberts
Mar 20, 2004·Developmental Cell·Thomas D Pollard
Jun 11, 2004·Annual Review of Biochemistry·Susanne M Rafelski, Julie A Theriot
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon

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Citations

Jun 21, 2011·PloS One·Pawel SzczesnyMarcin Grynberg
Oct 11, 2012·Bioscience, Biotechnology, and Biochemistry·Hanying YuXianzhi Jiang
Mar 8, 2016·Molecular and Biochemical Parasitology·Wansika PhadungsilRudi Grams
Aug 31, 2010·Molecular Reproduction and Development·Juan J Fraire-Zamora, Richard A Cardullo
Aug 5, 2009·Biophysical Journal·Jelena Stajic, Charles W Wolgemuth
Oct 9, 2016·Parasitology Research·Shuohan ZhengZhiyue Lv
Jan 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Alvaro Molina-CruzCarolina Barillas-Mury
May 6, 2021·The Korean Journal of Parasitology·Wansika Phadungsil, Rudi Grams

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