Structure of mistletoe lectin I from Viscum album in complex with the phytohormone zeatin

Biochimica Et Biophysica Acta
Arne MeyerChristian Betzel

Abstract

The crystal structure of mistletoe lectin I (ML-I) isolated from the European mistletoe Viscum album in complex with the most active phytohormone zeatin has been analyzed and refined to 2.54 A resolution. X-ray suitable crystals of ML-I were obtained by the counter-diffusion method using the Gel-Tube R crystallization kit (GT-R) onboard the Russian Service Module on the international space station ISS. High quality hexagonal bipyramidal crystals were grown during 3 months under microgravity conditions. Selected crystals were soaked in a saturated solution of zeatin and subsequently diffraction data were collected applying synchrotron radiation. A distinct F(o)-F(c) electron density has been found inside a binding pocket located in subunit B of ML-I and has been interpreted as a single zeatin molecule. The structure was refined to investigate the zeatin-ML-I interactions in detail. The results demonstrate the ability of mistletoe to protect itself from the host transpiration regulation by absorbing the most active host plant hormones as part of a defense mechanism.

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Citations

Feb 16, 2011·Journal of Experimental Botany·Ivo FrébortPetr Galuszka
Jul 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sachiko TakahashiYoshihiro Urade
Feb 13, 2016·International Journal of Molecular Sciences·Zheng ShiYong Tang
Jun 3, 2009·Cancer Letters·Bo LiuJin-ku Bao
Nov 9, 2011·Biochimica Et Biophysica Acta·Piotr H MałeckiArne Meyer
Apr 25, 2017·Critical Reviews in Biotechnology·Hedieh MalekzadMichael R Hamblin
Sep 17, 2009·Molecular BioSystems·Richard D Cummings
Jun 4, 2013·International Journal of Molecular Sciences·Irene Russo KraussFilomena Sica
Aug 8, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Arne MeyerChristian Betzel
Oct 9, 2019·International Journal of Biological Macromolecules·Ajay Kumar GautamKhem Chand Saini
Jun 16, 2021·Carbohydrate Research·Dixita ChettriAnil Kumar Verma

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