Structure of msj-1 gene in mice and humans: a possible role in the regulation of male reproduction

General and Comparative Endocrinology
Rosaria MeccarielloSilvia Fasano

Abstract

Msj-1 gene encodes a DnaJ protein highly expressed in spermatids and spermatozoa of both rodents and amphibians, possibly involved in vesicle fusion and protein quality control by means of interaction with heat shock proteins. We isolated and characterized the entire murine msj-1 gene and searched for putative msj-1-like genes into the human genome. Furthermore, ultrastructural localization of MSJ-1 was analyzed in mouse germ cells by immunogold electron microscopy. The analysis of murine msj-1 genomic sequence reveals that it is an intron less gene. Putative promoter region was predicted within the 600 bp upstream the transcription start site. In mouse, msj-1 maps on chromosome 1, into an intronic region of UDP glucuronosyl-transferase 1 family cluster. At ultrastructural level, MSJ-1 marks the developing acrosomic vesicle and the sperm centriolar region. A blast search against the human genome database revealed two closed regions (Ha and Hb) on human chromosome 2 having high nucleotide identity with murine msj-1 coding region. Similarly to mouse, in human both regions map into an intronic region of UDP glycosyl-transferase 1 family polypeptide A cluster (ugt1a@). A significant ORF encoding a putative DnaJ protein of 145 aa wa...Continue Reading

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Citations

Dec 11, 2013·International Journal of Endocrinology·Giovanna CacciolaGilda Cobellis
Sep 8, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Rosanna ChianeseRosaria Meccariello
May 22, 2009·Annals of the New York Academy of Sciences·Riccardo PierantoniSilvia Fasano
Mar 7, 2021·Ageing Research Reviews·Abdoulaye DianeMohammed Dehbi
Sep 12, 2017·Journal of Proteome Research·Christine CarapitoCharles Pineau

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