Structure of OxyAtei : completing our picture of the glycopeptide antibiotic producing Cytochrome P450 cascade

FEBS Letters
Kristina Haslinger, Max J Cryle

Abstract

Cyclization of glycopeptide antibiotic precursors occurs in either three or four steps catalyzed by Cytochrome P450 enzymes. Three of these enzymes have been structurally characterized to date with the second enzyme along the pathway, OxyA, escaping structural analysis. We are now able to present the structure of OxyAtei involved in teicoplanin biosynthesis - the same enzyme recently shown to be the first active OxyA homologue. In spite of the hydrophobic character of the teicoplanin precursor, the polar active site of OxyAtei and its affinity for certain azole inhibitors hint at its preference for substrates with polar decorations. This article is protected by copyright. All rights reserved.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 18, 1998·Chemistry & Biology·A M van WageningenP J Solenberg
Sep 12, 2000·Bioinformatics·L Holm, J Park
Jun 2, 2001·Antonie van Leeuwenhoek·M SosioS Donadio
Jan 2, 2004·Microbiology·Margherita SosioStefano Donadio
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·E Krissinel, K Henrick
Dec 11, 2007·Bioorganic & Medicinal Chemistry Letters·Nina GeibJohn A Robinson
Aug 9, 2008·Organic & Biomolecular Chemistry·Katharina WoitheJohn A Robinson
Oct 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Max J Cryle, Ilme Schlichting
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström
Oct 27, 2010·Archives of Biochemistry and Biophysics·Max J CryleIlme Schlichting
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Apr 17, 2013·Biotechnology and Applied Biochemistry·Xiaofeng YuRebecca C Wade
Apr 24, 2014·Organic & Biomolecular Chemistry·Patrick C SchmartzJohn A Robinson
Nov 2, 2014·Chembiochem : a European Journal of Chemical Biology·Kristina HaslingerMax J Cryle
Feb 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kirsty J McLeanMarco A van den Berg

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Citations

Oct 19, 2016·Scientific Reports·Madeleine PeschkeMax J Cryle
Dec 13, 2016·Chemical Reviews·Man-Cheng TangYi Tang
Nov 17, 2020·Natural Product Reports·Wolfgang Hüttel, Michael Müller

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