Structure of Parvovirus B19 Decorated by Fabs from a Human Antibody

Journal of Virology
Yingyuan SunMichael G Rossmann

Abstract

Parvovirus B19, one of the most common human pathogens, is a small DNA virus that belongs to the Parvoviridae As a result of previous infections, antibodies to B19 are present in most adults. B19 has a strong tropism to erythroid progenitor cells and is able to cause a series of medical conditions, including fifth disease, arthritis, myocarditis, hydrops fetalis, and aplastic crisis. No approved vaccine is currently available for B19, and there is a lack of structural characterization of any B19 epitopes. Here we present the first cryo-electron microscopy (cryo-EM) structure of a B19 virus-like particle (VLP) complexed with the antigen-binding fragment (Fab) of a human neutralizing antibody, 860-55D. A model was built into the 3.2-Å-resolution map, and the antigenic residues on the surface of the B19 capsid were identified. Antibody 860-55D bridges the capsid of B19 by binding to a quaternary structure epitope formed by residues from three neighboring VP2 capsid proteins.IMPORTANCE Parvovirus B19 is a common human pathogen and a particular threat to children, pregnant women, and patients with sickle cell disease or AIDS. Currently, neutralizing antibody is the most efficient treatment for acute B19 infections. Research on the a...Continue Reading

References

Sep 1, 1986·The Journal of Infectious Diseases·T ChorbaR Moir
Jan 22, 1987·The New England Journal of Medicine·A AnandB J Cohen
Jul 1, 1987·The Journal of Infectious Diseases·C H WoernleJ M Davison
Jul 30, 1987·The New England Journal of Medicine·G J KurtzmanN S Young
Aug 1, 1984·The Journal of Hygiene·M J AndersonB J Cohen
Oct 1, 1993·Science·K E BrownN S Young
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y TakahashiT Sasaki
Jan 12, 1999·Nature Medicine·C SummerfordR J Samulski
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Qing XieMichael S Chapman
Jun 5, 2003·Journal of Structural Biology·Joseph A Mindell, Nikolaus Grigorieff
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bärbel KaufmannMichael G Rossmann
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jan 22, 2005·Virology·Bärbel KaufmannRobert Seckler
May 14, 2005·Journal of Structural Biology·Christian SulowayBridget Carragher
Sep 21, 2005·Circulation·Uwe KühlHeinz-Peter Schultheiss
Dec 1, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·T LowinS Modrow
Jul 25, 2006·Journal of Structural Biology·Guang TangSteven J Ludtke
May 30, 2008·Journal of Virology·Bärbel KaufmannMichael G Rossmann
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 2, 2010·The New England Journal of Medicine·Claus-Thomas BockReinhard Kandolf
May 18, 2012·Journal of Virology·Brittney L GurdaMavis Agbandje-McKenna
Aug 9, 2012·Pediatric Cardiology·Kimberly M MolinaDavid P Nelson
Sep 25, 2012·Journal of Structural Biology·Sjors H W Scheres
Dec 21, 2013·Methods in Molecular Biology·Fei Guo, Wen Jiang
Jan 28, 2016·Nature·S PillayJ E Carette
Aug 19, 2016·Journal of Virology·Lindsey J OrgantiniSusan Hafenstein

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Citations

Apr 24, 2019·Viruses·Mario MietzschMavis Agbandje-McKenna
Apr 22, 2020·Viral Immunology·Shanan N EmmanuelMavis Agbandje-McKenna
Jul 22, 2019·Viruses·Elisabetta Manaresi, Giorgio Gallinella
Sep 22, 2021·Journal of Tropical Pediatrics·Vinícius Silva MonteiroMichele Souza Bastos

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