Structure of pre-S2 N- and O-linked glycans in surface proteins from different genotypes of hepatitis B virus

The Journal of General Virology
S SchmittWolfram H Gerlich

Abstract

The middle-sized (M) surface proteins of hepatitis B virus (HBV) and other orthohepadnaviruses contain a conserved N-glycan in their pre-S2 domain, which is essential for the secretion of viral particles. Recently, we also found O-glycans in the pre-S2 domain of M protein from woodchuck hepatitis virus (WHV) and HBV genotype D. Since the O-glycosylation motif is not conserved in all genotypes of HBV, the glycosylation patterns of HBV genotypes A and C were analysed. Pre-S2 (glyco)peptides were released from HBV-carrier-derived HBV subviral particles by tryptic digestion, purified by reversed-phase HPLC and identified by amino acid and amino-terminal sequence analysis as well as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Pre-S2 N-glycans were characterized by anion-exchange chromatography, methylation analysis and on-target sequential exoglycosidase digestions in combination with MALDI-TOF-MS, demonstrating the presence of partially sialylated diantennary complex-type oligosaccharides in all genotypes examined. Pre-S2 O-glycans were characterized by on-target sequential exoglycosidase digestions in combination with MALDI-TOF-MS. The pre-S2 domain of M protein and, to a minor exte...Continue Reading

References

Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·V Bruss, D Ganem
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y ItohM Mayumi
Jul 1, 1987·Archives of Biochemistry and Biophysics·B L Gillece-CastroA L Burlingame
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A MiyanoharaK Matsubara
Nov 1, 1984·Journal of Virology·K H HeermannW H Gerlich
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·T M BlockR A Dwek
Jan 1, 1997·Journal of Viral Hepatitis·W F Carman
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·R E LanfordR Rico-Hesse
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·N Dejucq, B Jégou
Aug 2, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M D YoungUNKNOWN US Hepacare Study Group
Aug 10, 2001·Infectious Diseases in Obstetrics and Gynecology·S A Gall
Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Philippe GriponChristiane Guguen-Guillouzo
May 3, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Stephan SchaeferWolfram H Gerlich
Jun 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ting ZhangWen-Zhe Ho
Aug 14, 2003·Journal of Virology·Dieter GlebeWolfram H Gerlich
Jan 13, 2004·Reviews in Medical Virology·Angeline Bartholomeusz, Stephan Schaefer

❮ Previous
Next ❯

Citations

Sep 12, 2012·Medical Microbiology and Immunology·Reinhild Prange
Nov 6, 2009·Glycoconjugate Journal·Elizabeth Higgins
Oct 2, 2007·Protein Expression and Purification·Sophie OttoneOdile Letourneur
Dec 1, 2006·Biochimica Et Biophysica Acta·Hildegard Geyer, Rudolf Geyer
May 17, 2008·Journal of Viral Hepatitis·M L Op den BrouwA M Woltman
Mar 20, 2013·Journal of Medical Virology·Paula E FlorianNorica Branza-Nichita
Jun 25, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R A A Pondé
Sep 1, 2009·Viruses·Stephanie Schädler, Eberhard Hildt
May 1, 2018·Expert Review of Proteomics·David J Harvey
Dec 19, 2007·Cellular Microbiology·Corinna M LeistnerDieter Glebe
Jun 10, 2020·Cells·Mihaela-Olivia DobricaNorica Branza-Nichita
Aug 2, 2020·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kiyoaki ItoHisashi Narimatsu
Jun 2, 2021·The Journal of Clinical Investigation·Tsung-Yu TsaiShie-Liang Hsieh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.