Structure of proline iminopeptidase from Xanthomonas campestris pv. citri: a prototype for the prolyl oligopeptidase family

The EMBO Journal
F J MedranoF X Gomis-Rüth

Abstract

The proline iminopeptidase from Xanthomonas campestris pv. citri is a serine peptidase that catalyses the removal of N-terminal proline residues from peptides with high specificity. We have solved its three-dimensional structure by multiple isomorphous replacement and refined it to a crystallographic R-factor of 19.2% using X-ray data to 2.7 A resolution. The protein is folded into two contiguous domains. The larger domain shows the general topology of the alpha/beta hydrolase fold, with a central eight-stranded beta-sheet flanked by two helices and the 11 N-terminal residues on one side, and by four helices on the other side. The smaller domain is placed on top of the larger domain and essentially consists of six helices. The active site, located at the end of a deep pocket at the interface between both domains, includes a catalytic triad of Ser110, Asp266 and His294. Cys269, located at the bottom of the active site very close to the catalytic triad, presumably accounts for the inhibition by thiol-specific reagents. The overall topology of this iminopeptidase is very similar to that of yeast serine carboxypeptidase. The striking secondary structure similarity to human lymphocytic prolyl oligopeptidase and dipeptidyl peptidase ...Continue Reading

References

Apr 1, 1992·Protein Engineering·D L OllisJ Schrag
May 15, 1992·Biochemical and Biophysical Research Communications·P EhrenfreundG Kreil
Feb 1, 1991·Hypertension·K KoharaC M Ferrario
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S K BurleyW N Lipscomb
Feb 1, 1985·Antimicrobial Agents and Chemotherapy·H Osada, K Isono
Dec 1, 1987·Journal of Pharmacobio-dynamics·T YoshimotoD Tsura
Oct 20, 1995·Science·C M FraserJ C Venter
Sep 20, 1994·Biochemistry·J A EndrizziS J Remington
Oct 15, 1994·FEMS Microbiology Letters·R P AllakerJ M Hardie
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Jan 1, 1993·Protein Engineering·G J Barton

❮ Previous
Next ❯

Citations

Jul 12, 2002·Medicinal Research Reviews·Claudiu T SupuranBrian W Clare
Feb 24, 2007·Applied Microbiology and Biotechnology·B Geueke, H-P E Kohler
Jan 18, 2005·Molecular Genetics and Genomics : MGG·Daniëlle E J W BastenPeter J Schaap
Aug 4, 1999·Immunology Today·I De MeesterS Scharpé
Jan 5, 2001·The Biochemical Journal·K M FukasawaN Ota
May 7, 2010·The Journal of Biological Chemistry·Min LiThang K Chiu
Jun 7, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Chao Yu YangShan Ho Chou
Dec 18, 2008·Applied and Environmental Microbiology·Arati V Patankar, Juan E González
Jan 18, 2002·Infection and Immunity·J M GoldsteinJ Travis
Jan 19, 2010·BMC Genomics·Mengjin LiuRoland J Siezen
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·K HåkanssonC G Miller
Apr 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael EngelHans Brandstetter
Oct 6, 2007·Biochimica Et Biophysica Acta·Misugi UrajiTadashi Hatanaka
Aug 8, 2007·Journal of Molecular Biology·Pier Federico GherardiniMichael J E Sternberg
Jan 25, 2007·Journal of Biochemistry and Molecular Biology·Hiroaki NagaiHidenori Ichijo
Feb 19, 2009·FEMS Microbiology Reviews·Arati V Patankar, Juan E González
Feb 15, 2012·Biochemical and Biophysical Research Communications·Urszula SzawłowskaWiesław Bielawski
Mar 28, 2017·Biochemistry·Jacqueline L Naffin-OlivosDagmar Ringe
Oct 13, 2018·Protein Science : a Publication of the Protein Society·Polytimi S DimitriouKonstantin Denessiouk
Jul 5, 2005·The Journal of Biological Chemistry·Peter GoettigJeong-Sun Kim
Jan 24, 2004·Molecular Genetics and Genomics : MGG·H OginoH Ishikawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.