PMID: 39756Aug 1, 1979

Structure of pyridine nucleotide transhydrogenase from Azotobacter vinelandii

European Journal of Biochemistry
G VoordouwE F van Bruggen

Abstract

1. Pyridine nucleotide transhydrogenase of Azotobacter vinelandii purified by affinity chromatography consists of a mixture of polydisperse rods at neutral pH. No other structures are seen by electron microscopy. 2. At high pH (8.5--9.0) the rods depolymerize. Complete depolymerization can be achieved in 0.1 M Tris-Cl pH 9.0. The depolymerized enzyme has a molecular weight of 421000 (sedimentation equilibrium), its sedimentation coefficient s20, w = 15 S and its Stokes' radius Rs = 7 nm. Since gel electrophoresis in the presence of sodium dodecyl sulphate shows that transhydrogenase consists of a single polypeptide chain of molecular weight (54 +/- 2) X 10(3) it follows that the depolymerized enzyme has an octameric quaternary structure. We propose that this octamer serves as the functional monomeric unit ('unimer') from which the polymeric form of transhydrogenase is constructed. 3. Gel filtration and sucrose gradient centrifugation studies of cell-free extracts from A. vinelandii show the unimer to be the predominant active species.

References

Nov 1, 1981·European Journal of Biochemistry·W Slobbe, G Voordouw
Oct 1, 1982·European Journal of Biochemistry·G VoordouwP Zabel
Apr 5, 1983·European Journal of Biochemistry·G VoordouwA P Themmen
Aug 19, 2015·Frontiers in Microbiology·Sebastiaan K SpaansServé W M Kengen

Citations

Jan 1, 1976·Advances in Protein Chemistry·H EisenbergE Reisler
Oct 24, 1977·Biochemical and Biophysical Research Communications·B Höjeberg, J Rydström
Nov 15, 1975·European Journal of Biochemistry·T W BrestersC Veeger
Oct 14, 1972·Journal of Molecular Biology·D D LouieJ D McLean
Jan 1, 1972·Methods in Enzymology·K WeberM Osborn
Mar 1, 1957·Biochimica Et Biophysica Acta·V N SCHUMAKER, H K SCHACHMAN
Mar 1, 1964·Biochemistry·D A YPHANTIS

Related Concepts

Tromethamine
Centrifugation
Electron Microscopy, Diagnostic
Gel Chromatography
Polymers
Protein Conformation
Electron Microscopy
Sedimentation Procedure
NNT
Rod Photoreceptors

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