PMID: 3845122Jan 20, 1985

Structure of ribosome-bound messenger RNA as revealed by enzymatic accessibility studies

Journal of Molecular Biology
C W Kang, C R Cantor

Abstract

Digestion with ribonuclease T2 has been used to study the size of poly(U) protected by ribosome binding. Several different preparations of ribosomes all appear to cover 49 nucleotides of message; however, there are two partially accessible internal nuclease cleavage sites, which yield, ultimately, fragments 20, 16 and 13 nucleotides in length. Curiously, the site between fragments of length 20 and 16 is accessible to RNase T2 but not to the several much smaller RNases. Arguments based on the quantitative pattern of cleavage and comparisons with previous studies lead to the conclusion that the 20-mer is the 5' fragment, while 13-mer (which is lost the moment it is cleaved from the 16-mer) is the 3' fragment. Both ribosome-bound tRNAs appear to contact only the 16-mer. The presence of the two internal cleavage sites fits nicely with recent electron microscopic data suggesting that mRNA forms a loop around the 30 S subunit.

References

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Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·S S Thach, R E Thach
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Citations

Jul 1, 1993·World Journal of Microbiology & Biotechnology·C M Arraiano
Dec 1, 1990·Experientia·E J Murgola
Jul 1, 1991·Biochimie·I N ShatskyV D Vasiliev
Apr 1, 1992·Biochimie·E N Trifonov
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G NilssonA von Gabain
Dec 1, 1991·Molecular Microbiology·J Dreher, H Matzura
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Charles L Turnbough, Robert L Switzer
Jan 21, 2000·Journal of Theoretical Biology·T LesnikC Reiss
Oct 3, 1989·Biochemistry·G J Murakawa, D P Nierlich
Sep 28, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Leah B ShawKelvin H Lee
Jul 28, 1995·The Journal of Biological Chemistry·A P PotapovK H Nierhaus

Related Concepts

Bacillus intermedius secreted ribonuclease
Molecular Sieve Chromatography
Endoribonucleases
Poly U
Ribosomes
Poly(A) Tail
RNA, Transfer, Amino Acyl
Macromolecular Compounds

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